ISSN:
1573-4919
Keywords:
T-tubules
;
monoclonal antibodies
;
freeze fracture
;
Mg++ATPase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Summary A microsomal fraction consisting of membranes of transverse tubule origin has been purified by a modification of the calcium-loading procedure initially described by Rosemblatt et al. (J Biol Chem 256:8140–8, 1981). Enzymatic analysis of this fraction shows an enrichment of the vesicles in the Mg++ATPase (basal) activity characteristic of the T-tubules and an absent or very low Ca++-dependant ATPase activity. Stereological analysis of freeze fracture replica of the membranes in the purified fraction indicates that they have a very low density of particles in their P faces and lack the structural manifestation of the caveolae typical of the sarcolemma. Immunological analysis performed with monoclonal antibodies prepared against purified T-tubule and sarcoplasmic reticulum membranes define some T-tubule specific antigens and confirm the morphological and biochemical data regarding the origin and purity of the Ttubule preparation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00421083
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