ISSN:
1432-072X
Keywords:
Candida boidinii
;
Methanol
;
Assimilation
;
Incorporation of
;
Formaldehyde
;
Ribose-Phosphate-Cycle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Hexose phosphate synthetase activity was found in cell-free extracts of methanol-grown Candida boidinii. Incubation of this crude extract with 14C-formaldehyde and D-ribose-5-phosphate leads to incorporation of radioactivity into fructose-and glucose phosphates. Cells grown on glucose lack the hexose phosphate synthetase activity. No hydroxypyruvate reductase activity, the key enzyme of the serine pathway was found. These results indicate that during growth of C. boidinii on methanol, cell constituents are made by a sugar phosphate pathway similar in concept, if not in absolute molecular detail, to the ribose phosphate cycle in C1-metabolizing bacteria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00403055
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