ISSN:
1432-072X
Keywords:
Key words Physical map
;
Chromosome
;
Archaea
;
Methanogens
;
Methanobacterium thermoautotrophicum
;
Marburg
;
Methanobacterium wolfei
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A physical and genetic map of the chromosome of Methanobacterium wolfei was constructed by using pulsed-field gel electrophoresis of restriction fragments generated by digestion with NotI and NheI. The chromosome was found to be circular and 1,729 kb in size. Twenty-eight genes were mapped to specific restriction enzyme fragments by performing hybridization experiments with gene probes from various Methanobacterium strains. The genomic map obtained was compared with the updated genomic map of Methanobacterium thermoautotrophicum Marburg. In spite of major restriction pattern dissimilarities, the overall genetic organization seemed to be conserved between the genomes of the two strains. In addition, the two rRNA operons of strain Marburg were precisely mapped on the chromosome, and it was shown that they are transcribed in the same direction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00305354
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