ISSN:
1432-2048
Keywords:
Brassica
;
Cryoprotection
;
Freeze-thaw damage (thylakoids)
;
Frost hardiness
;
Membrane permeability
;
Spinacia
;
Thylakoid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have isolated protein fractions from cold-acclimated, frost-hardy cabbage (Brassica oleracea L.) and spinach (Spinacia oleracea L.) leaves which protect isolated thylakoids from non-hardy spinach against mechanical membrane rupture during an in-vitro freeze-thaw cycle. No protective activity was found in similar preparations from non-hardy leaves. The proteins protected the membranes from damage by reducing their solute permeability during freezing and by increasing their expandability during thawing. The proteins act by increasing the resistance of the membranes against the osmotic stress to which they are exposed during a freeze-thaw cycle. In the absence of cryoprotectants this stress results in membrane rupture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00198794
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