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  • 1
    Electronic Resource
    Electronic Resource
    Heteroplasmy of the chloroplast genome of Medicago sativa L. cv ‘Regen S’' confirmed by sequence analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 685-690 
    Details
    ISSN: 1432-2242
    Keywords: Heteroplasmy ; Chloroplast DNA ; Medicago sativa L. ; Sequence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The heteroplasmy of chloroplast DNA (cpDNA) observed in Medicago sativa L., which involves the presence (type B) or absence (type A) of an Xba I restriction site, was examined using closed fragments covering the variable XbaI site from type-A and type-B cpDNA. The 6.2-kb PstI fragment of DNA from type-A cpDNA (−XbaI) and from type-B cpDNA (+XbaI) was cloned into pUC19 plasmids. EcoRI fragments bearing the variable XbaI site from the type-A and type-B 6.2-kb PstI fragments were subcloned into pUC19. DNA sequences of both types of the 696-bp EcoRI fragments were determined and computer-assisted analysis of the sequence data carried out. Type-A cpDNA was found to differ from type-B cpDNA by 1 base, a G to T conversion, which results in a non-recognition site for XbaI in the type-A cpDNA. The sequence difference was in a non-coding region. Cloning and sequencing of the fragments verified the individual identity of the type-A and type-B cpDNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224063
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  • 2
    Electronic Resource
    Electronic Resource
    Restriction endonuclease studies on the chloroplast and mitochondrial DNAs of alfalfa (Medicago sativa L.) protoclones (1986)
    Springer
    Plant molecular biology 6 (1986), S. 331-338 
    Details
    ISSN: 1573-5028
    Keywords: alfalfa ; chloroplast DNA ; Medicago sativa L. ; mitochondrial DNA ; protoclone ; protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alfalfa protoclones were regenerated from the mesophyll protoplasts of two cloned source plants (parents), RS-K1 and RS-K2, initiated from Regen S seed. Because of the high frequency of karyotypic upset previously observed in these plants, chloroplast DNAs (cpDNA) from 23 protoclones and mitochondrial DNAs (mtDNA) from 20 protoclones were examined by restriction endonuclease analysis in order to assess recombination in their cytoplasmic genomes. Seven and four endonucleases were separately used for cpDNA and mtDNA analysis, respectively. Data were consistent with no, or a low frequency of, major sequence rearrangements in either the chloroplast or the mitochondrial genomes as a result of protocloning. However, two types of cpDNA were detected in the 23 protoclones, with only one protoclone possessing the cpDNA type of the cloned parental populations sampled. Possible explanations include a preferential selection during protocloning for one of two parental cpDNA types, an in planta sorting out of cpDNA types in the parental material or both.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034940
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    Paper (German National Licenses)
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