ISSN:
1432-2242
Keywords:
Key words RFLP markers
;
Fine-mapping
;
Blast resistance
;
Pyramiding
;
Marker-assisted selection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior ricevarieties by marker-aided selection (MAS).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051395
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