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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 20 (1998), S. 113-118 
    ISSN: 1573-0603
    Keywords: End-labeling ; GBS ; Mapping ; Restriction enzyme mapping ; Tn917
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two pieces of data are needed to fully map the location of a transposon inserted in a plasmid, the site of insertion and the transposon's orientation. Both of these parameters can be determined from a map of restriction sites, which can be derived by end-probing. Like restriction mapping, end-probing reveals the distance between restriction sites on a plasmid. In contrast to restriction mapping, end-probing unambiguously reveals the order of those restriction sites. End-probing is similar to end-labeling, except that the uncertainties inherent in the radiolabeling reaction (and the problems of working with radionucleotides) are avoided. In this paper we discuss the use of end-probing as a means to map insertions of transposon Tn917 into the Streptococcus agalactiae plasmid pGB354
    Type of Medium: Electronic Resource
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