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  • Mapping
  • AGAMOUS
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  • 1
    Electronic Resource
    Electronic Resource
    Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 66-72 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sorghum bicolor ; Simple sequence repeats (SSRs) ; Polymorphism ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of these loci were isolated by screening sorghum genomic AG-enriched libraries with labelled poly(AG)/poly(CT), the other three were derived from database searches. In order to explore the degree of polymorphism detectable in this species by this type of molecular marker, the SSR markers were tested on nine inbred lines of S. bicolor of different geographic origin. PCR analysis on acrylamide gels revealed a high degree of polymorphism (δT=0.80). One locus, in particular, allowed the identification of all of the nine inbred lines used in our study. Seven of these SSR markers were mapped, using an existing sorghum RFLP map.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050533
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  • 2
    Electronic Resource
    Electronic Resource
    CaMADS1, a MADS box gene expressed in the carpel of hazelnut (1998)
    Springer
    Plant molecular biology 38 (1998), S. 1147-1160 
    Details
    ISSN: 1573-5028
    Keywords: hazelnut ; Corylus avellana ; MADS box genes ; flower development ; ovule ; AGAMOUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hazelnut (Corylus avellana L.) is a species of economic interest that shows a peculiar floral biology. Unlike most of the angiosperms, which produce ovules during floral development such that they are ready for pollen at anthesis, hazelnut ovary development is delayed and triggered by compatible pollination. In order to elucidate the mechanisms regulating this unusual process and the role of the MADS box genes in ovary development, a cDNA library from pollinated styles of hazelnut was screened with a mixture of MADS box genes from different plant species. CaMADS1 (Corylus avellana MADS box), a floral-specific MADS box gene, was isolated, and characterized as belonging to the sub-family of the AGAMOUS genes. Northern blot, RT-PCR analyses and in situ hybridization experiments show a precise correlation between ovary development and CaMADS1 expression, indicating a role of this MADS box gene in the processes of floral organogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006022524708
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis and mapping of two genes encoding maize glutathione S-transferases (GST I and GST II) (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 535-539 
    Details
    ISSN: 1617-4623
    Keywords: Glutathione S-transferase ; Maize ; Gene expression ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which (Gst I, II andIII) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism including a “null” variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA from “null” and “plus” genotypes, usingGst I- andGst II-specific probes, indicated that theGst I gene controls the expression of the two major GST isoforms expressed in roots.Gst I andGst II were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized.Gst I was localized on the long arm of chromosome 8, while two putativeGst II loci were mapped to chromosomes 8 (70 cM fromGst I) and 10, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02423448
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