Publication Date:
2004-01-17
Description:
Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (ManS), that makes the beta-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase-like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in beta-glycan formation in plants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dhugga, Kanwarpal S -- Barreiro, Roberto -- Whitten, Brad -- Stecca, Kevin -- Hazebroek, Jan -- Randhawa, Gursharn S -- Dolan, Maureen -- Kinney, Anthony J -- Tomes, Dwight -- Nichols, Scott -- Anderson, Paul -- New York, N.Y. -- Science. 2004 Jan 16;303(5656):363-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, 7300 NW 62nd Avenue, Johnston, IA 50131, USA. Kanwarpal.Dhugga@Pioneer.Com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14726589" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Arabidopsis/enzymology/genetics
;
Catalytic Domain
;
Cellulose/biosynthesis
;
Cyamopsis/*enzymology/genetics
;
Databases, Nucleic Acid
;
Expressed Sequence Tags
;
Gene Expression
;
Gene Library
;
*Genes, Plant
;
Glucosyltransferases/chemistry/*genetics/metabolism
;
Golgi Apparatus/enzymology
;
Mannans/*biosynthesis/metabolism
;
Mannose/metabolism
;
Mannosyltransferases/chemistry/*genetics/isolation & purification/*metabolism
;
Molecular Sequence Data
;
Multigene Family
;
Oryza/enzymology/genetics
;
Phylogeny
;
Plants, Genetically Modified
;
Protein Structure, Tertiary
;
Seeds/*enzymology
;
Soybeans/genetics
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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