ISSN:
1573-5087
Keywords:
gas chromatography
;
nitrogen/phosphorus detector
;
1-aminocyclopropane-1-carboxylic acid (ACC)
;
soil flooding
;
root-shoot communication
;
xylem sap
;
environmental stress
;
Lycopersicon esculentum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The Lizada and Yang method, commonly used for analyzing 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of the plant hormone ethylene, is subject to interference and lacks internal standards. The use of combined gas chromatography-mass spectrometry (GC-MS) overcomes these shortcomings but the method is expensive and unavailable to many laboratories. We describe an alternative physico-chemical method using a capillary column gas chromatograph fitted with a standard nitrogen/phosphorus detector. After forming the N-benzoyl n-propyl derivative, measurements of ACC concentrations in extracts of leaves and in xylem sap of tomato plants using the nitrogen/phosphorus detector were within 10% of those obtained by GC-MS. Concentrations in plants grown in well-drained soil were approximately 0.16 nmol g−1 fresh weight (leaves) and 0.04–0.01 mmol m−3 (sap). Flooding the soil for 48–72 h increased these values approximately 9-fold.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00024842
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