ISSN:
1573-8264
Keywords:
isoenzyme pattern
;
Lycopersicon esculentum
;
quantitative variation
;
F1 hybrids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Expression of esterase isoenzymes in 1272 seeds was used to estimate hybridity of Lycopersicon esculentum Mill. Individual seeds (440) of the parental cultivars taken from different experimental stations in Bulgaria were also analysed. The banding patterns were obtained by means of vertical block electrophoresis in polyacrylamide gels. It was established that quantitative variation of locus Est-1 can be applied to prove hybridity of F1 tomato seeds. This marker is related to the genetic nature of tomatoes and is not the result of the environmental influence. The reason for this conclusion is the fact that the isoenzymes of the Est-1 locus are indicative for hybridity determination of all examined seeds taken from different regions in Bulgaria. Use of this locus is to be recommended for both its universality and the fact that the reagents for esterase isoenzymes staining are not expensive.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1001887218557
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