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  • Aircraft Stability and Control  (6)
  • Lycopersicon esculentum  (2)
  • 1995-1999  (8)
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  • 1995-1999  (8)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Improved Screening of cDNAs Generated by mRNA Differential Display Enables the Selection of True Positives and the Isolation of Weakly Expressed Messages (1997)
    Springer
    Plant molecular biology reporter 15 (1997), S. 236-245 
    Details
    ISSN: 1572-9818
    Keywords: Differential display ; Lycopersicon esculentum ; Ethylene-responsive ; Low abundance mRNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The high percentage of false positives generated by differential display (as high as 85%) has previously limited the potential of the method. This report describes an efficient methodology that enables false positives to be discarded prior to cloning, via reverse Northern analysis. This first step of the screening also allows the detection of putative low abundance differential clones. Following cloning, a second reverse Northern combined with partial DNA sequencing and RT-PCR detection allows isolation of all differential cDNAs including very low abundance clones. Use of the sequential screening procedure described here led to the isolation of novel tomato genes responding to the plant hormone ethylene while minimising labor and materials input.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007482318668
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Er5, a tomato cDNA encoding an ethylene-responsive LEA-like protein: characterization and expression in response to drought, ABA and wounding (1997)
    Springer
    Plant molecular biology 35 (1997), S. 847-854 
    Details
    ISSN: 1573-5028
    Keywords: differential display ; drought stress ; ethylene inducible ; LEA protein ; Lycopersicon esculentum ; RT-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the isolation by differential display of a novel tomato ethylene-responsive cDNA, designated ER5. RT-PCR analysis of ER5 expression revealed an early (15 min) and transient induction by ethylene in tomato fruit, leaves and roots. ER5 mRNA accumulated during 2 h of ethylene treatment and thereafter underwent a dramatic decline leading to undetectable expression after 5 h of treatment. The full-length cDNA clone of 748 bp was obtained and DNA sequence analysis showed strong homologies to members of the atypical hydrophobic group of the LEA protein family. The predicted amino acid sequence shows 67%, 64%, 64%, and 61% sequence identity with the tomato Lemmi9, soybean D95-4, cotton Lea14-A, and resurrection plant pcC27-45 gene products, respectively. As with the other members of this group, ER5 encodes a predominantly hydrophobic protein. Prolonged drought stress stimulates ER5 expression in leaves and roots, while ABA induction of this ethylene-responsive clone is confined to the leaves. The use of 1-MCP, an inhibitor of ethylene action, indicates that the drought induction of ER5 is ethylene-mediated in tomato roots. Finally, wounding stimulates ER5 mRNA accumulation in leaves and roots. Among the Lea gene family this novel clone is the first to display an ethylene-regulated expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005860302313
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Wavelet Applications for Flight Flutter Testing (1999)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: Wavelets present a method for signal processing that may be useful for analyzing responses of dynamical systems. This paper describes several wavelet-based tools that have been developed to improve the efficiency of flight flutter testing. One of the tools uses correlation filtering to identify properties of several modes throughout a flight test for envelope expansion. Another tool uses features in time-frequency representations of responses to characterize nonlinearities in the system dynamics. A third tool uses modulus and phase information from a wavelet transform to estimate modal parameters that can be used to update a linear model and reduce conservatism in robust stability margins.
    Keywords: Aircraft Stability and Control
    Type: CEAS/AIAA/ICASE/NASA Langley International Forum on Aeroelasticity and Structural Dynamics 1999; Pt. 1; 393-402; NASA/CP-1999-209136/PT1
    Format: application/pdf
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  • 4
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    Worst-Case Flutter Margins from F/A-18 Aircraft Aeroelastic Data (1997)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: An approach for computing worst-case flutter margins has been formulated in a robust stability framework. Uncertainty operators are included with a linear model to describe modeling errors and flight variations. The structured singular value, micron, computes a stability margin which directly accounts for these uncertainties. This approach introduces a new method of computing flutter margins and an associated new parameter for describing these margins. The micron margins are robust margins which indicate worst-case stability estimates with respect to the defined uncertainty. Worst-case flutter margins are computed for the F/A-18 SRA using uncertainty sets generated by flight data analysis. The robust margins demonstrate flight conditions for flutter may lie closer to the flight envelope than previously estimated by p-k analysis.
    Keywords: Aircraft Stability and Control
    Type: NASA/TM-97-207564 , NAS 1.15:207564 , AIAA Paper 97-1266 , Structures, Structural Dynamics and Materials Conference; Apr 01, 1997; Orlando, FL; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Wavelet Filtering to Reduce Conservatism in Aeroservoelastic Robust Stability Margins (1998)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Wavelet analysis for filtering and system identification was used to improve the estimation of aeroservoelastic stability margins. The conservatism of the robust stability margins was reduced with parametric and nonparametric time-frequency analysis of flight data in the model validation process. Nonparametric wavelet processing of data was used to reduce the effects of external desirableness and unmodeled dynamics. Parametric estimates of modal stability were also extracted using the wavelet transform. Computation of robust stability margins for stability boundary prediction depends on uncertainty descriptions derived from the data for model validation. F-18 high Alpha Research Vehicle aeroservoelastic flight test data demonstrated improved robust stability prediction by extension of the stability boundary beyond the flight regime.
    Keywords: Aircraft Stability and Control
    Type: NASA/TM-1998-206545 , NAS 1.15:206545 , H-2222 , AIAA Paper 98-1896 , Structures, Structural Dynamics and Materials Conference; Apr 20, 1998 - Apr 23, 1998; Long Beach, CA; United States
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  • 6
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    Estimation of Modal Parameters Using a Wavelet-Based Approach (1997)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Modal stability parameters are extracted directly from aeroservoelastic flight test data by decomposition of accelerometer response signals into time-frequency atoms. Logarithmic sweeps and sinusoidal pulses are used to generate DAST closed loop excitation data. Novel wavelets constructed to extract modal damping and frequency explicitly from the data are introduced. The so-called Haley and Laplace wavelets are used to track time-varying modal damping and frequency in a matching pursuit algorithm. Estimation of the trend to aeroservoelastic instability is demonstrated successfully from analysis of the DAST data.
    Keywords: Aircraft Stability and Control
    Type: NASA/TM-97-206300 , NAS 1.15:206300 , H-2214 , Atmospheric Flight Mechanics Conference; Aug 08, 1997 - Aug 11, 1997; New Orleans, LA; United States
    Format: application/pdf
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  • 7
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    Utilizing Flight Data to Update Aeroelastic Stability Estimates (1997)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Stability analysis of high performance aircraft must account for errors in the system model. A method for computing flutter margins that incorporates flight data has been developed using robust stability theory. This paper considers applying this method to update flutter margins during a post-flight or on-line analysis. Areas of modeling uncertainty that arise when using flight data with this method are investigated. The amount of conservatism in the resulting flutter margins depends on the flight data sets used to update the model. Post-flight updates of flutter margins for an F/A-18 are presented along with a simulation of on-line updates during a flight test.
    Keywords: Aircraft Stability and Control
    Type: Atmospheric Flight Mechanics Conference; Aug 01, 1997; New Orleans, LA; United States
    Format: text
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  • 8
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    Wavelet Applications for Flight Flutter Testing (1999)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Wavelets present a method for signal processing that may be useful for analyzing responses of dynamical systems. This paper describes several wavelet-based tools that have been developed to improve the efficiency of flight flutter testing. One of the tools uses correlation filtering to identify properties of several modes throughout a flight test for envelope expansion. Another tool uses features in time-frequency representations of responses to characterize nonlinearities in the system dynamics. A third tool uses modulus and phase information from a wavelet transform to estimate modal parameters that can be used to update a linear model and reduce conservatism in robust stability margins.
    Keywords: Aircraft Stability and Control
    Type: H-2364 , International Forum on Aeroelasticity and Structural Dynamics; Jun 22, 1999 - Jun 25, 1999; Williamsburg, VA; United States
    Format: application/pdf
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