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  • Lunar and Planetary Science and Exploration  (5)
  • maize  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Sequence, identification and characterization of cDNAs encoding two different members of the 18 kDa heat shock family of Zea mays L. (1991)
    Springer
    Plant molecular biology 16 (1991), S. 699-711 
    Details
    ISSN: 1573-5028
    Keywords: heat shock ; heat shock cDNAs ; maize ; small heat shock proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heat-shocked maize seedlings (cv. Oh43) synthesize a characteristic set of heat-shock proteins (hsps) which include an 18 kDa family containing at least six major isoelectric variants. A cDNA library was constructed from poly(A)+ RNAs isolated from the radicles of heat-shocked maize seedlings and screened with a DNA fragment from the theoretical open reading frame of a putative Black Mexican Sweet maize hsp 18 genomic clone. Two clones, cMHSP18-3 and cMHSP18-9, were isolated, and the RNA transcripts generated from them were translated into proteins which immunoreact with antibodies directed against the maize 18 kDa hsps and exhibit the same electrophoretic characteristics as two different members of the 18 kDa hsp family. Nucleotide sequence analyses of the cDNAs in these clones reveal that their 5′ and 3′ untranslated regions exhibit 33–34% identity and that their protein encoding regions share 93% identity. The deduced amino acid sequences of these clones show 90% identity, and the apparent molecular masses and isoelectric points of these proteins agree with those established for two different 18 kDa hsps, numbered 3 and 6. This report substantiates that at least two of the 18 kDa hsps in maize are products of different but related genes. Moreover, it establishes that transcripts for these proteins accumulate during heat shock and that both their nucleotide and deduced amino acid sequences share extensive similarities with the class VI small hsps in soybean and with transcripts expressed during meiosis in Lilium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023434
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway (1996)
    Springer
    Plant molecular biology 30 (1996), S. 269-279 
    Details
    ISSN: 1573-5028
    Keywords: carotenoid biosynthesis ; endosperm ; gene ; maize ; phytoene desaturase ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To study regulation of the plastid-localized maize carotenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized. The DNA sequence of the maize Pds cDNA was determined and compared with available dicot Pds genes. The deduced PDS protein, estimated at 64.1 kDa (unprocessed), had a dinucleotide binding domain and conserved regions characteristic of other carotene desaturases. Alignment of available PDS sequences from distantly related organisms suggests that Pds has potential as a phylogenetic tool. By use of heterologous complementation in Escherichia coli, maize PDS was shown to catalyze two desaturation steps converting phytoene to ζ-carotene. RFLP (restriction fragment length polymorphism) mapping was used to place Pds on chromosome 1S near viviparous5 (vp5), and RT-PCR (reverse-transcriptase polymerase chain reaction) analysis indicated reduced Pds transcript in vp5 mutant relative to normal endosperm. Other phytoene-accumulating mutant endosperms, vp2 and white3 (w3), showed no difference in Pds transcript accumulation as compared with normal endosperm counterparts. RT-PCR analysis of Pds transcript accumulation in developing endosperm showed Pds was constitutively expressed. Therefore, endosperm carotenogenesis is not regulated by increasing the level of Pds transcripts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020113
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  • 3
    Electronic Resource
    Electronic Resource
    The independent stage-specific expression of the 18-kDa heat shock protein genes during microsporogenesis in Zea mays L (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 15-26 
    Details
    ISSN: 0192-253X
    Keywords: Heat shock protein ; maize ; mi-crosporogenesis ; gametogenesis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The small (18-kDa) heat shock proteins (hsps) of maize are encoded by a complex multigene family. In a previous report, we described the genetic information from cDNAs encoding two different members of the family. In this communication, we report the isolation and characterization of cDNA and genomic clones encoding information for a third member of this hsp family (c/gMHSP18-1). DNA fragments containing nucleotide sequences common to, or specific for, each of these characterized 18-kDa genes were prepared and used as probes to assess the expression of these genes during microsporogenesis and development of the gametophyte in an inbred line of maize (Oh43). Our results demonstrate (1) that mRNA transcripts encoding the 18-kDa hsps are expressed and/or accumulate during microsporogenesis, and (2) that genes encoding two of the characterized 18-kDa hsps are expressed and/or accumulate independently, in a stage-specific manner during microsporogenesis. These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response. © 1993Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020140104
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  • 4
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    Report on the Loss of the Mars Polar Lander and Deep Space 2 Missions (2000)
    In:  CASI
    Details
    Publication Date: 2013-08-31
    Description: NASA's Mars Surveyor Program (MSP) began in 1994 with plans to send spacecraft to Mars every 26 months. Mars Global Surveyor (MGS), a global mapping mission, was launched in 1996 and is currently orbiting Mars. Mars Surveyor '98 consisted of Mars Climate Orbiter (MCO) and Mars Polar Lander (MPL). Lockheed Martin Astronautics (LMA) was the prime contractor for Mars Surveyor '98. The Jet Propulsion Laboratory (JPL), California Institute of Technology, manages the Mars Surveyor Program for NASA's Office of Space Science. MPL was developed under very tight funding constraints. The combined development cost of MPL and MCO, including the cost of the two launch vehicles, was approximately the same as the development cost of the Mars Pathfinder mission, including the cost of its single launch vehicle. The MPL project accepted the challenge to develop effective implementation methodologies consistent with programmatic requirements.
    Keywords: Lunar and Planetary Science and Exploration
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Theoretical Calculations on Sediment Transport on Titan, and the Possible Production of Streamlined Forms (2005)
    In:  CASI
    Details
    Publication Date: 2017-10-02
    Description: The Cassini Imaging Science System (ISS) has been returning images of Titan, along with other Saturnian satellites. Images taken through the 938 nm methane window see down to Titan's surface. One of the purposes of the Cassini mission is to investigate possible fluid cycling on Titan. Lemniscate features shown recently and radar evidence of surface flow prompted us to consider theoretically the creation by methane fluid flow of streamlined forms on Titan. This follows work by other groups in theoretical consideration of fluid motion on Titan's surface.
    Keywords: Lunar and Planetary Science and Exploration
    Type: Lunar and Planetary Science XXXVI, Part 2; LPI-Contrib-1234-Pt-2
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  • 6
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    First Results of the Athena Microscopic Imager Investigation (2004)
    In:  CASI
    Details
    Publication Date: 2018-06-11
    Description: The Athena science payload on the Mars Exploration Rovers (MER) includes the Microscopic Imager (MI). The MI is a fixed-focus camera mounted on an extendable arm, the Instrument Deployment Device (IDD). The MI acquires images at a spatial resolution of 30 microns/pixel over a broad spectral range (400 - 700 nm). The MI uses the same electronics design as the other MER cameras but its optics yield a field of view of 31 x 31 mm across a 1024 x 1024 pixel CCD image. The MI acquires images using only solar or skylight illumination of the target surface. A contact sensor is used to place the MI slightly closer to the target surface than its best focus distance (about 69 mm), allowing concave surfaces to be imaged in good focus. Coarse focusing (approx. 2 mm precision) is achieved by moving the IDD away from a rock target after contact is sensed. The MI optics are protected from the Martian environment by a retractable dust cover. This cover includes a Kapton window that is tinted orange to restrict the spectral bandpass to 500 - 700 nm, allowing crude color information to be obtained by acquiring images with the cover open and closed. The MI science objectives, instrument design and calibration, operation, and data processing were described by Herkenhoff et al. Initial results of the MI experiment on both MER rovers ('Spirit' and 'Opportunity') are described below.
    Keywords: Lunar and Planetary Science and Exploration
    Type: Lunar and Planetary Science XXXV: Mars Missions; LPI-Contrib-1197
    Format: text
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  • 7
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    Distant Secondary Craters and Age Constraints on Young Martian Terrains (2005)
    In:  CASI
    Details
    Publication Date: 2017-10-02
    Description: Are small (less than approx. 1 km diameter) craters on Mars and the Moon dominated by primary impacts, by secondary impacts of much larger primary craters, or are both primaries and secondaries significant? This question is critical to age constraints for young terrains and for older terrains covering small areas, where only small craters are superimposed on the unit. If the martian rayed crater Zunil is representative of large impact events on Mars, then the density of secondaries should exceed the density of primaries at diameters a factor of ~1000 smaller than that of the largest contributing primary crater. On the basis of morphology and depth/diameter measurements, most small craters on Mars could be secondaries. Two additional observations (discussed below) suggest that the production functions of Hartmann and Neukum predict too many primary craters smaller than a few hundred meters in diameter. Fewer small, high-velocity impacts may explain why there appears to be little impact regolith over Amazonian terrains. Martian terrains dated by small craters could be older than reported in recent publications.
    Keywords: Lunar and Planetary Science and Exploration
    Type: Lunar and Planetary Science XXXVI, Part 13; LPI-Contrib-1234-Pt-13
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  • 8
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    Basaltic Ring Structures as an Analog for Ring Features in Athabasca Valles, Mars (2005)
    In:  CASI
    Details
    Publication Date: 2017-10-02
    Description: Basaltic ring structures (BRSs) are enigmatic, quasi-circular landforms in eastern Washington State that were first recognized in 1965. They remained a subject of geologic scrutiny through the 1970 s and subsequently faded from the spotlight, but recent Mars Orbiter Camera (MOC) images showing morphologically similar structures in Athabasca Valles, Mars, have sparked renewed interest in BRSs. The only known BRSs occur in the Channeled Scabland, a region where catastrophic Pleistocene floods from glacial Lake Missoula eroded into the Miocene flood basalts of the Columbia Plateau. The geologic setting of the martian ring structures (MRSs) is similar; Athabasca Valles is a young channel system that formed when catastrophic aqueous floods carved into a volcanic substrate. This study investigates the formation of terrestrial BRSs and examines the extent to which they are appropriate analogs for the MRSs in Athabasca Valles.
    Keywords: Lunar and Planetary Science and Exploration
    Type: Lunar and Planetary Science XXXVI, Part 10; LPI-Contrib-1234-Pt-10
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