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  • Liquid chromatography  (6)
  • Springer  (6)
  • 1980-1984  (6)
  • 1982  (6)
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Keywords
Publisher
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  • 1980-1984  (6)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Temperature effects on liquid chromatographic absorbance stability at low wavelengths (1982)
    Springer
    Chromatographia 16 (1982), S. 162-165 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Ultra-violet detection ; Temperature effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This paper is concerned with our investigation into the effects of temperature on the liquid chromatography absorbance baseline. Measurements were made to quantify the effects in terms of wavelength and solvents. Five solvents commonly used in HPLC were investigated, methanol, propanol, hexane, heptane and acetonitrile. The alcoholic solvents were found to have a high absorbance sensitivity to temperature at wavelengths around their ‘UV cut off’ points. Possible mechanisms for this effect are discussed and include solvent impurity, density changes and inter-molecular bonding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258889
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Electrochemical detector with a 20 nl volume for micro-columns liquid chromatography (1982)
    Springer
    Chromatographia 16 (1982), S. 265-266 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Short micro bore columns ; Small volume electrochemical detector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A flow-through two electrode wall-jet cell with a platinum measuring electrode and a cell volume of 20 nl has been designed and evaluated. It has been used to detect phenols by reversed phase liquid chromatography using short micro bore columns. The linear dynamic range between the measured current and the concentration is greater than 103 (1.5×10−7−5×10−4 mol/l) and the minimum analyzable amount was found to be 10 pg for pyrocatechol. A negligible broadening in the detector permits the use of micro columns down to 0.5 mm internal diameter, packed with 5 μm particles, without any substantial distortion of eluted zones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258916
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  • 3
    Electronic Resource
    Electronic Resource
    An investigation of 3μm reverse-phase octadecylsilane packing materials for high-performance liquid chromatography (1982)
    Springer
    Chromatographia 15 (1982), S. 657-659 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Reverse-phase ; 3 μm packing materials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The efficiency of high-performance liquid chromatography columns packed with 5 μm and 3 μm reverse-phase octadecylsilane packing materials has been evaluated using four test mixtures. Both stopped flow and valve injection have been used and typical efficiencies are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279495
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  • 4
    Electronic Resource
    Electronic Resource
    Chromatographic analysis of allergens (1982)
    Springer
    Chromatographia 16 (1982), S. 216-218 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Size exclusion ; Biological extracts ; Allergens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Extracts of insect, mite, fungal and mammalian origin, known to provoke an allergic response in man, have been examined and fractionated by high-performance size exclusion chromatography. The mild conditions employed for separation have permitted direct immunological assessment of the fractions obtained, and recognition of the biologically important components. These are being isolated for further study. This chromatographic procedure has proved to be of value for assessing different extracts from the same source material as potential reference preparations. It can also be employed as a sensitive method for identification of extracts derived from a variety of sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258905
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  • 5
    Electronic Resource
    Electronic Resource
    Chemically-bonded chiral column packings for high-performance liquid chromatography (1982)
    Springer
    Chromatographia 16 (1982), S. 224-227 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Chiral bonded phases ; Aminoacid racement resolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary N-Protected aminoacids have been chemically bonded to aminopropylated silica gel, and the resulting chiral phases have been applied to the LG resolution of racemic mixtures of polar compounds. Best results were obtained with surfacebound N-formyl-isoleucine and N-formylvaline, with which baseline resolutions of a range of aminoacid and aminoalcohol derivatives were achieved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258907
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  • 6
    Electronic Resource
    Electronic Resource
    Reaction detector with three dimensional coiled open tubes in HPLC (1982)
    Springer
    Chromatographia 15 (1982), S. 403-408 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Reaction detector ; Colorimetric detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A reaction detector with non-segmented flow in open tubes as reaction track is described. To minimize peak broadening the open tubes are arranged in a three dimensional coiled structure by knitting. At low volume flow rates the h-values in these open tubes are independent of flow rate and are significantly lower than predicted by theory. The applicability of this reactor for classical colorimetric detection with corrosive reagents is shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261598
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