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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 5 (1982), S. 271-281 
    ISSN: 0148-7280
    Keywords: marmosets ; cow zonae pellucidae ; active immunization ; radioimmunoassay ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A double antibody radioimmunoassay technique is described for the measurement of anti-zona antibodies in the peripheral plasma of marmosets actively immunized against intact cow zonae pellucidae. The method has been shown to be a reliable, repeatable indicator of antibody titer, enabling direct comparison of the response obtained between marmosets. This is the first report of a procedure describing the active immunization of a primate against zona antigens, and the first time a radioimmunoassay technique has been used to monitor profiles of anti-cow zona antibody production following active immunization. The method should prove to be a useful tool in the evaluation of zona antigens as agents for immunocontraception.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 41-47 
    ISSN: 0148-7280
    Keywords: zona pellucida ; antibody ; sperm receptors ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Mouse zonae pellucidae contain receptors for sperm throughout their structure since spermatozoa will bind to both the inner and outer surfaces of isolated zona fragments. Antibodies raised against mechanically isolated mouse zonae pellucidae were only capable of suppressing sperm binding to the outer zona surface in association with the formation of a precipitate in this region.These results indicate that such antisera are not capable of interacting directly with the sperm receptors on the zona pellucida but rely upon the less efficient mechanism of steric hindrance to prevent sperm from gaining access to the sperm binding sites.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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