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  • 1
    Electronic Resource
    Electronic Resource
    The early development of the swim bladder and certain adjacent parts in hemichromis bimaculata (1940)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 67 (1940), S. 1-57 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050670102
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  • 2
    Electronic Resource
    Electronic Resource
    Cytosolic phospholipase C activity: II. Relationship to concanavalin A-induced phosphatidylinositol-turnover in splenocytes (1994)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 56 (1994), S. 409-417 
    Details
    ISSN: 0730-2312
    Keywords: G proteins ; sodium metavanadate ; phorbol esters ; dexamethasone ; NaF ; trifluoperizine ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have described in the first paper the coupling betweencytosolic Giα and cytosolic PLC activity in a cell free preparation. In order to establish the functional significance of the cytosolic Giα coupled soluble PLC, we examined the effects of Dex, NaF, and trifluopeerizine (TEP) on concanavalin A(Con A)-induced PI-turnover in intact slenocytes and, in parallel, on soluble PLC activity in cytosol preparations. Vytosolic PLC activity was measured with [3H]PIP and [3H]PIP2 as substrates. (1) The con A-induced increase (2-4 fold) in Pl-turnover in intact splenocytes was paralleled by an 1.2-5-fold increase in soluble PLC activity in vitro. Con A administration also increased cytosolic Giα immunoreactivity 3-6-fold as expected if cytosolic Giα was coupled to soluble PLC activation. (2) DEX (10-7 M), administered 6 h prior to Con A administration inbited the Con A-induced increase in Pl-turnover in intact splenocytes. This was paralleled by DEX inhibition of the Con A-induced increase in soluble PLC activity measured in vitro and cytosolic Giα imunoreactivity. (3) We have demonstrated in the first paper that NaF and TEP inhibited soluble PLC activity. Here we show that NaF and TFP inhibited the Con A-induced increase in PI-turnover extending the similarities between soluble PLC activity and Con A- Stimulated PLC Activity in intact splenocytes. (4) In order to examine Whether or not the Con A-induced PLC activity and Con A-stimulated PLC activity in intact splenocytes. (4) In order to examine Whether or not the Con A-induced PLC was similar to PLCγ, we measured PI-turnover induced by Con A or BaVO3 in combination with DEX and PMA. Whereas the Con A-induced PI-turnover was significantly inhibited (40-60%) by DEX, the NaVO3 -induced PI-turnover was not affected by DEX. The Con A-induced PI-turnover was not affected by PMA (50nM), But the NaVO3-induced Pi-turnover was increased over 2-fold PMA (50nM), suggesting that the Con A-induced PLC in intact splenocytes is different from NaVO3-induced PLC. Based on these results a model for the sequential activation of substrate-specific PLCs in splenocyte by mitogen is presented.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240560317
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  • 3
    Electronic Resource
    Electronic Resource
    Cytosolic phospholipase C activity: I. Evidence for coupling with cytosolic guanine nucleotide-binding protein, Giα (1994)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 56 (1994), S. 397-408 
    Details
    ISSN: 0730-2312
    Keywords: antisensense oligonucleotides ; pertussis toxin ; splenocytes ; Nb2 cells ; Giα ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: In a previous report we shwed that glucocorticoed inhibition of cytosolic PLC activity correlated with a reduction in cytosolic Giα levels, suggesting that there may be a functional relationship between cytosolic PLC and cytosolic Giα. In order to establish the nature of the coupliing between cytosolic Giα and cytosolic PLC we examined the effects of Protein activators, and inhibitors on cytosolic PLC activity from rat spenocytes and the rat lymphoma cell line Nb 2, with [3H] PI and [3H]PIP2 as substrates. (1) Neither GTP nor its nonhydrolyzable analogue, GTPγS, at 100 μm had any effect on the calcium stimulated as well as the basal PLC activity. (2) Howevr, affinity purified antibodies to Giα1 and Giα2 inhibited soluble PLC activity, by 85% and 55%, respectively, with PI as substrate; with PIP2 as substrate, soluble PLC activity was inhibited 50-70% by antibodies to Gi1, whereas antibodies to Gi2 had little effect. (3)Administration of Giα1 antisense oligonucleotides to splenocytes for 48 h produced 25-40% decrease in cytosolic Giα1 levels compared to control. The soluble PLC activity with both PI and PIP2 as substrates was also reduced by 25-50% compared to control conditions. This suggest that cytosolic Giα is associated with the activation of splenocyte soluble PLC. (4) Pertussis toxin administered in vivo sugnificantly reduced cytosolic Giα immunoreactivity and soluble PLC activiry when PI was used as substrate, providing additional evidence that cytosolic Giα is associated with the activation of splencyte soluble PLC. (5) Another agent that has beeen used extensively to define G-protein coupled processes is NaF/AlCl3. NaF(4mM; with or without AlCl3 inhibited soluble PLC activity with PIP2 as substrate, in contrast ot the stimulatory effect that has been reported in the activation of membrane PLC. 6) because NaF can act as a protein phosphatase inhibitor, we also tested the effects of trifluoperzine (50 μm, TFP), an inhibitor of protein phosphatase 2B; TFP (50 μm) signigicantly inhibited soluble PLC activity PI was used as substrate. These results suggest a direct involvement of cytosolic Giα in the activation of soluble PLC form splenocytes. Other questions pertaining to the functional significance, the nature, and possible substrate preference of the splenocyte Giα coupled PLC is addressed in the second paper.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240560316
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  • 4
    Electronic Resource
    Electronic Resource
    The early development of Haemichromis bimaculata, with special reference to factors determining the embryonic axis (1930)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 49 (1930), S. 579-619 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Haemichromis bimaculata is a tropical teleost fish which will produce eggs practically throughout the year at intervals of from three to four weeks. These eggs are of suitable size and character for embryological study, the features of special interest so far discovered being as follows: (1) The egg is oval; (2) it is attached to a substratum by its side, the blastopore being at one end; (3) the embryo always tends to develop along the side opposite that originally next to the substratum.Results obtained by reorienting the eggs previous to cleavage, and by centrifuging them, seem to show that the relation of the embryonic axis to the egg is determined either previous to laying or very soon afterward, possibly by the relation to the substratum, and is not subsequently affected by gravity or other known factors. There may be some tendency for the first cleavage plane and the sagittal plane of the embryo to coincide, but such coincidence is not at all constant or exact.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1050490210
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  • 5
    Electronic Resource
    Electronic Resource
    Mitochondrial membrane biogenesis: Characterization and use of pet mutants to clone the nuclear gene coding for subunit V of yeast cytochrome c oxidase (1984)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 24 (1984), S. 229-242 
    Details
    ISSN: 0730-2312
    Keywords: cytochrome oxidase ; subunit V ; nuclear genes ; assembly ; Saccharomyces cerevisiae ; pet mutants ; mitochondria ; biogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A nuclear pet mutant of Saccharomyces cerevisiae that is defective in the structural gene for subunit V of cytochrome c oxidase has been identified and used to clone the subunit V gene (COX5) by complementation. This mutant, E4-238 [24], and its revertant, JM110, produce variant forms of subunit V. In comparison to the wild-type polypeptide (Mr = 12,500), the polypeptides from E4-238 and JM110 have apparent molecular weights of 9,500 and 13,500, respectively. These mutations directly alter the subunit V structural gene rather than a gene required for posttranslational processing or modification of subunit V because they are cis-acting in diploid cells; that is, both parental forms of subunit V are produced in heteroallelic diploids formed from crosses between the mutant, revertant, and wild type. Several plasmids containing the COX5 gene were isolated by transformation of JM28, a derivative of E4-238, with DNA from a yeast nuclear DNA library in the vector YEp13. One plasmid, YEp13-511, with a DNA insert of 4.8 kilobases, was characterized in detail. It restores respiratory competency and cytochrome oxidase activity in JM28, encodes a new form of subunit V that is functionally assembled into mitochondria, and is capable of selecting mRNA for subunit V. The availability of mutants altered in the structural gene for subunit V (COX5) and of the COX5 gene on a plasmid, together with the demonstration that plasmid-encoded subunit V is able to assemble into a functional holocytochrome c oxidase, enables molecular genetic studies of subunit V assembly into mitochondria and holocytochrome c oxidase.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240240305
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  • 6
    Electronic Resource
    Electronic Resource
    Secretion and pH-dependent self-processing of the pro-form of the Yarrowia lipolytica acid extracellular protease (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 1115-1125 
    Details
    ISSN: 0749-503X
    Keywords: Yarrowia lipolytica ; secretion ; pH ; extracellular protease ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The secretion and maturation of the acid extracellular protease (AXP) of the yeast Yarrowia lipolytica have been characterized using antiserum raised against this enzyme. A 42 kDa pro-enzyme form of AXP was identified from lysates of radiolabelled Y. lipolytica cells and found to contain no N-linked carbohydrate moieties. Using pulse-chase immune precipitation it was demonstrated that the AXP precursor was secreted into the extracellular medium where, under conditions of low pH, it underwent autocatalytic activation forming the mature enzyme. Conversion of the AXP pro-form in the presence of the protease inhibitor pepstatin indicated that an intramolecularly-catalysed reaction mechanism was involved in AXP maturation. Further evidence supporting the role of autocatalytic processing came from the side-chain specificity of mature AXP towards the oxidized B-chain of insulin. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Three-dimensional tomographic reconstruction in high voltage electron microscopy (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 193-205 
    Details
    ISSN: 0741-0581
    Keywords: HVEM ; EM ; Three-dimensional reconstruction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The methodology is described, as developed at the Albany NIH Biotechnological High Voltage Electron Microscope Resource, for the three-dimensional reconstruction of objects in thick sections. The reconstructions are obtained from projection sets which are recorded by high voltage electron microscopy. The different steps of the procedure are illustrated in detail, using the cilium reconstruction as an example.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060210
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