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  • Analytical Chemistry and Spectroscopy  (4)
  • Life and Medical Sciences  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Human sperm chromosomes obtained from hamster eggs after sperm capacitation in TEST-yolk buffer (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 11 (1985), S. 253-259 
    Details
    ISSN: 0148-7280
    Keywords: TEST-yolk buffer ; capacitation ; human sperm chromosomes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Human sperm chromosomes were obtained after capacitation with TES-Tris (TEST) yolk buffer and fusion with Syrian hamster eggs. Semen samples could be stored at 4°C for 3 days and remain functional in the assay system. The efficiency of TEST yoik buffer for obtaining karyotypes was as good as, or greater than, the efficiency of standard BWW medium containing human serum albumin.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120110304
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the first cell cycle in the cross between hamster eggs and human sperm (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 23 (1989), S. 299-308 
    Details
    ISSN: 0148-7280
    Keywords: human sperm chromatin ; S-phase ; DNA synthesis ; bromodeoxyuridine ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Golden hamster eggs fused with human sperm were pulsed with bromodeoxyuridine to determine the timing of S-phase and the length of the first ceil cycie in this hybrid cross. Fused eggs were fixed and pronuclei scored for incorporation of the thymidine analogue detected by indirect immunofiuorescence. Although S-phase started synchronously 3-3.5 hr after coincuba-tion of sperm and eggs, its duration was variable such that two-cell stages appeared at 16 hr while a proportion of pronuclei was still engaged in DNA synthesis. Unlike rodent sperm chromatin, human sperrn chromatin was able to participate in DNA synthesis well before its maturation into a fully developed pronucleus. Human sperm chromatin appears able to function under conditions different in several respects from those in human eggs.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120230307
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  • 3
    Electronic Resource
    Electronic Resource
    Mass spectra of thiophene- and pyrrole-2-carboxyaldehyde condensation products prepared from ephedrine derivatives (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 24 (1989), S. 1022-1024 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210241109
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  • 4
    Electronic Resource
    Electronic Resource
    The endoplasmic reticulum and calcium storage (1990)
    New York, NY : Wiley-Blackwell
    BioEssays 12 (1990), S. 527-531 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Calcium storage is one of the functions commonly attributed to the endoplasmic reticulum (ER) in non-muscle cells. Several recent studies have added support to this concept. Analysis of reticuloplasm, the luminal ER content, has shown that it contains several proteins (reticuloplasmins) which are prospective calcium storage proteins. One of these, calreticulin, is also present in the sarcoplasmic reticulum (SR). In sea urchin eggs, a calsequestrin-like protein has been clearly localised to the ER. The recent demonstration that the IP3 receptor, which has similarities with the calcium release channel in the SR is also localised in the ER membrane suggests that calcium stored in the ER is important for intracellular signalling. The alternative view, that the physiologically important calcium store is a specialised organelle, the calciosome, is not supported by these observations. Recent evidence also suggests that ER calcium might be important in ER structure and in the retention of the luminal ER proteins.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950121105
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  • 5
    Electronic Resource
    Electronic Resource
    13C NMR determination of the configuration of methyl-substituted double bonds in medium- and large-ring terpenoids (1986)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 24 (1986), S. 656-658 
    Details
    ISSN: 0749-1581
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 13C NMR spectroscopy can be used to determine the configuration of methyl-substituted double bonds in medium- and large-ring terpenoid natural products. A number of examples from our investigations in the germacranolide field are presented, and clearly show that if the resonance for the vinylic methyl group appears at a value greater than 20 ppm the double bond has a (Z)-configuration whereas if the value is less than 20 ppm an (E)-configuration is present. Literature examples of other classes of compounds are also cited to illustrate the generality of this method.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260240804
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  • 6
    Electronic Resource
    Electronic Resource
    1H and 13C NMR analyses of amiodarone, desethylamiodarone and desoxoamiodarone (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 482-493 
    Details
    ISSN: 0749-1581
    Keywords: Amiodarone ; Desethylamiodarone ; Desoxoamiodarone ; 1H NMR ; 13C NMR ; Selective INEPT ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 1H and 13C NMR spectra of amiodarone, a potent cardiac antiarrhythmic agent, desethylamiodarone, the main metabolite of amiodarone and desoxoamiodarone, a less toxic form of amiodarone, were assigned using a combination of NMR techniques. Methods applied include 1H-1H COSY, homonuclear Hartmann-Hahn relayed spectroscopy (HOHAHA), broadband decoupled 1H-13C chemical shift correlation, long-range 1H-13C chemical shift correlation and selective INEPT. This combination of NMR experiments has completed a molecular walk along the covalent framework of the cardiac drug molecules, and removed ambiguities in their 1H and 13C NMR assignments.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290513
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  • 7
    Electronic Resource
    Electronic Resource
    1H and 13C NMR study of the neomenthyl halides: A very high field proton shift in the iodide (1990)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 28 (1990), S. 659-660 
    Details
    ISSN: 0749-1581
    Keywords: NMR ; Neomenthyl halides ; Large upfield shifts ; Halogen substituent effect ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Complete analyses o the 1H NMR spectra of neomethyl iodide (1), the bromide (2) and the chloride (3) are reported. Of paticular interest is the very high-field resonance at -0.16 ppm in 1 which is assigned to H-4. The same poton in the bromide is also found upfield at 0.78 ppm. H-4 in these neomenthyl derivatives is tertiary and β-antipeiplanar to the halogen atom. A similar upfield shift has been reported previously for the tertiary proton in trans-9-iododecalin. The reasons or these unusual shifts are not readily apparent and the 13C NMR spectra of 1 and 2, which are reported here for the first time, provide no additional clues to explain the shifts.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260280719
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