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Progesterone facilitates chromosome instability (aneuploidy) in p53 null normal mammary epithelial cells (2000)

Medina, D. ; Ullrich, R. L. ; McCarthy, M. ; [weitere]

In: Other Sources

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Publikationsdatum: 2019-07-13

Beschreibung: Mammary epithelial cells from p53 null mice have been shown recently to exhibit an increased risk for tumor development. Hormonal stimulation markedly increased tumor development in p53 null mammary cells. Here we demonstrate that mammary tumors arising in p53 null mammary cells are highly aneuploid, with greater than 70% of the tumor cells containing altered chromosome number and a mean chromosome number of 56. Normal mammary cells of p53 null genotype and aged less than 14 wk do not exhibit aneuploidy in primary cell culture. Significantly, the hormone progesterone, but not estrogen, increases the incidence of aneuploidy in morphologically normal p53 null mammary epithelial cells. Such cells exhibited 40% aneuploidy and a mean chromosome number of 54. The increase in aneuploidy measured in p53 null tumor cells or hormonally stimulated normal p53 null cells was not accompanied by centrosome amplification. These results suggest that normal levels of progesterone can facilitate chromosomal instability in the absence of the tumor suppressor gene, p53. The results support the emerging hypothesis based both on human epidemiological and animal model studies that progesterone markedly enhances mammary tumorigenesis.

Schlagwort(e): Life Sciences (General)

Materialart: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology (ISSN 0892-6638); 14; 14; 2221-9

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Digitale Medien

Structure and control of assembly of cytoplasmic microtubules in normal and transformed cells (1976)

Fuller, G. M. ; Brinkley, B. R.

New York, N.Y. : Wiley-Blackwell

Journal of Supramolecular Structure 5 (1976), S. 497-514

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ISSN: 0091-7419

Schlagwort(e): Cytoplasmic microtubule complex ; calcium ; normal and transformed cells ; in vivo control ; effects of trypsin ; Life Sciences ; Molecular Cell Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Indirect immunoflurescence analyses using antibodieis directed against 6S tubulin have shown an elaborate cytoplasmic microtubule complex (CMTC) in nontransformed cells in culture. The CMTC is strikingly altered in cells that have been transformed spontaneously by viruses or by chemicals. Assembly of microtubules in vitro and in vivo is markedly inhibited in the presence of elevated levels of calcium. Alteration of the surface of normal cells by brief treatment with low concentrations of trypsin initiate a rapid breakdown of cytoplasmic microtubules. Finally, a hypothesis is presented relating microtubule assembly and surface membrane modulation suggesting that calcium is the primary modulating signal.

Zusätzliches Material: 19 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jss.400050407

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