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    Contribution of dietary oxalate to urinary oxalate excretion (2001)
    In:  Other Sources
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    Publication Date: 2019-07-13
    Description: BACKGROUND: The amount of oxalate excreted in urine has a significant impact on calcium oxalate supersaturation and stone formation. Dietary oxalate is believed to make only a minor (10 to 20%) contribution to the amount of oxalate excreted in urine, but the validity of the experimental observations that support this conclusion can be questioned. An understanding of the actual contribution of dietary oxalate to urinary oxalate excretion is important, as it is potentially modifiable. METHODS: We varied the amount of dietary oxalate consumed by a group of adult individuals using formula diets and controlled, solid-food diets with a known oxalate content, determined by a recently developed analytical procedure. Controlled solid-food diets were consumed containing 10, 50, and 250 mg of oxalate/2500 kcal, as well as formula diets containing 0 and 180 mg oxalate/2500 kcal. Changes in the content of oxalate and other ions were assessed in 24-hour urine collections. RESULTS: Urinary oxalate excretion increased as dietary oxalate intake increased. With oxalate-containing diets, the mean contribution of dietary oxalate to urinary oxalate excretion ranged from 24.4 +/- 15.5% on the 10 mg/2500 kcal/day diet to 41.5 +/- 9.1% on the 250 mg/2500 kcal/day diet, much higher than previously estimated. When the calcium content of a diet containing 250 mg of oxalate was reduced from 1002 mg to 391 mg, urinary oxalate excretion increased by a mean of 28.2 +/- 4.8%, and the mean dietary contribution increased to 52.6 +/- 8.6%. CONCLUSIONS: These results suggest that dietary oxalate makes a much greater contribution to urinary oxalate excretion than previously recognized, that dietary calcium influences the bioavailability of ingested oxalate, and that the absorption of dietary oxalate may be an important factor in calcium oxalate stone formation.
    Keywords: Life Sciences (General)
    Type: Kidney international (ISSN 0085-2538); 59; 1; 270-6
    Format: text
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  • 2
    Electronic Resource
    Electronic Resource
    A new approach to determination of the nucleotide sequence of a viral RNADiscussion paper presented by C. Weissmann.This project is supported by grants from the Schweizerische Nationalfonds (no. 4347, 4589, and 5272 to C.W.; FN 5209-3 to Prof. A. Tissières; and FN 5205-3 to Prof. R. Epstein); the British Science Research Council and NATO (to J.H.); the Jane Coffin Childs Fund (no. 243 to C.W.); and the American Cancer Society Inc. (no. PF 356-A to J.D. and PF 432 to H.M.G.). (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 74 (1969), S. 197-204 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A Qβ RNA segment was produced in vitro by means of a synchronized minus-strand-directed synthesis using purified Qβ replicase, a reaction known to yield infectious Qβ RNA. Arguments are presented that a 5.5S product obtained by incubation for 30 seconds at 20°C corresponds to the 5′-terminal region of Qβ RNA. After obtaining and sequencing 26 T1 and 21 pancreatic RNase products, we established 3′ nearest neighbors by using products labeled with only one nucleoside [α-32P]triphosphate at a time. The ordering of the oligonucleotides was facilitated by observing the time of their appearance in products from incubations of 5, 10, 15, 20, and 25 seconds. The enzymatic product consists of 160 nucleotides; no known initiation triplet appears before the sixtieth nucleotide. There is no indication that the coat protein cistron starts within the RNA segment analyzed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040740420
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