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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 313 (1971), S. 411-421 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Russian Abstract.
    Abstract: It seems advisable to investigate polycondensation processes extended systematically from low to high conversion by carefully chosen model systems, considering the statistical data available on the processes themselves and the relationship between structure and properties of the resulting polymers. In a previous paper (Rev. Gén. Caoutchouc Plastiques, Éd. Plastiques, 4, (1967) 111) dealing with conventional crosslinking agents, the possibility was suggested to perform polycondensation processes approaching more closely the classical random distribution. This can be achieved with symmetrical monomers having functional groups well spaced out to avoid substitution effects and cyclisation. Cyclisation is also rendered more difficult by greater stiffness of the structure of the monomers. This paper shows by some examples that, in favourable cases, the deviation from the classical random distribution can be reduced to the limits of experimental error, thus enabling the physical properties to be compared with the results of comparatively simple model calculations.
    Notes: Es ist ratsam, Polykondensationsprozesse an äußerst sorgfältig gewählten Modellsystemen zu studieren, und zwar systematisch von niedrigeren nach höheren Umsätzen, und indem man die Kenntnis der Prozesse selbst, der resultierenden Struktur der Produkte, und deren strukturgebundenen physikalischen Eigenschaften gleichzeitig auf statistischer Basis vertieft. In einem früheren Bericht (Rev. Gén. Caoutchouc Plastiques, Éd. Plastiques, 4, 1967, 111) über bekannte organische und anorganische Netzformer wurde auf die Möglichkeit hingewiesen, Polykondensationssysteme zu entwickeln, die den reinen klassischen Zufallsverteilungen in viel besserer Näherung folgen. Hierzu wählt man symmetrische Monomere mit räumlich weit auseinanderliegenden Funktionen, um Substitutionseffekte und Ringschlüsse weitgehend zu vermeiden; weiterhin werden Ringschlüsse auch durch Steifheit der Monomerstruktur sehr erschwert. In diesem Vortrag wird an einigen Beispielen belegt, daß man so tatsächlich die Abweichungen vom klassischen (cyclenfreien) Zufallsmodell in günstigen Fällen erwartungsgemäß bis zur Grenze der Unmeßbarkeit zurückdrängen kann, und daß sich das deshalb lohnt, weil man dann physikalische Eigenschaften der Produkte mit relativ einfachen Modellrechnungen vergleichen kann.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 3 (1969), S. 165-174 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Factor assays of normal citrated plasma following incubation with nonleaching heparinized surfaces in high surface area-plasma volume ratios showed a marked pattern of reduction in factor IX activity, with lesser depression of II and X. No or modest effect on other factors was observed. In particular, no significant adsorption of factor XII (Hageman) by either cationic or anionic surfaces, was found. No consistent pattern of effects was seen with a group of strongly anionic surfaces, analogous to the sulfate and sulfamate groupings of the heparin molecule. However, certain members of this class showed profound effects on factor XI.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2011-08-24
    Description: To investigate the endoplasmic reticulum (ER) Ca(2+) stores in plant cells, we generated tobacco (Nicotiana tabacum; NT1) suspension cells and Arabidopsis plants with altered levels of calreticulin (CRT), an ER-localized Ca(2+)-binding protein. NT1 cells and Arabidopsis plants were transformed with a maize (Zea mays) CRT gene in both sense and antisense orientations under the control of an Arabidopsis heat shock promoter. ER-enriched membrane fractions from NT1 cells were used to examine how altered expression of CRT affects Ca(2+) uptake and release. We found that a 2.5-fold increase in CRT led to a 2-fold increase in ATP-dependent (45)Ca(2+) accumulation in the ER-enriched fraction compared with heat-shocked wild-type controls. Furthermore, after treatment with the Ca(2+) ionophore ionomycin, ER microsomes from NT1 cells overproducing CRT showed a 2-fold increase in the amount of (45)Ca(2+) released, and a 2- to 3-fold increase in the amount of (45)Ca(2+) retained compared with wild type. These data indicate that altering the production of CRT affects the ER Ca(2+) pool. In addition, CRT transgenic Arabidopsis plants were used to determine if altered CRT levels had any physiological effects. We found that the level of CRT in heat shock-induced CRT transgenic plants correlated positively with the retention of chlorophyll when the plants were transferred from Ca(2+)-containing medium to Ca(2+)-depleted medium. Together these data are consistent with the hypothesis that increasing CRT in the ER increases the ER Ca(2+) stores and thereby enhances the survival of plants grown in low Ca(2+) medium.
    Keywords: Life Sciences (General)
    Type: Plant physiology (ISSN 0032-0889); Volume 126; 3; 1092-104
    Format: text
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  • 4
    Publication Date: 2011-08-24
    Description: We show that the tightly regulated tetracycline-sensitive Top10 promoter system (Weinmann et al. Plant J. 1994, 5, 559-569) is functional in Arabidopsis thaliana. A pure breeding A. thaliana line (JL-tTA/8) was generated which expressed a chimeric fusion of the tetracycline repressor and the activation domain of Herpes simplex virus (tTA), from a single transgenic locus. Plants from this line were crossed with transgenics carrying the ER-targeted green fluorescent protein coding sequence (mGFP5) under control of the Top10 promoter sequence. Progeny from this cross displayed ER-targeted GFP fluorescence throughout the plant, indicating that the tTA-Top10 promoter interaction was functional in A. thaliana. GFP expression was repressed by 100 ng ml-1 tetracycline, an order of magnitude lower than the concentration used previously to repress expression in Nicotiana tabacum. Moreover, the level of GFP expression was controlled by varying the concentration of tetracycline in the medium, allowing a titred regulation of transgenic activity that was previously unavailable in A. thaliana. The kinetics of GFP activity were determined following de-repression of the Top10:mGFP5 transgene, with a visible ER-targeted GFP signal appearing from 24 to 48 h after de-repression.
    Keywords: Life Sciences (General)
    Type: The Plant journal : for cell and molecular biology (ISSN 0960-7412); Volume 21; 6; 579-88
    Format: text
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  • 5
    Publication Date: 2019-07-19
    Description: International Space Station (ISS) assembly complete ushered a new era focused on utilization of this state-of-the-art orbiting laboratory to advance science and technology research in a wide array of disciplines, with benefits to Earth and space exploration. ISS enabling capability for research in cellular and molecular biology includes equipment for in situ, on-orbit analysis of biomolecules. Applications of this growing capability range from biomedicine and biotechnology to the emerging field of Omics. For example, Biomolecule Sequencer is a space-based miniature DNA sequencer that provides nucleotide sequence data for entire samples, which may be used for purposes such as microorganism identification and astrobiology. It complements the use of WetLab-2 SmartCycler"TradeMark", which extracts RNA and provides real-time quantitative gene expression data analysis from biospecimens sampled or cultured onboard the ISS, for downlink to ground investigators, with applications ranging from clinical tissue evaluation to multigenerational assessment of organismal alterations. And the Genes in Space-1 investigation, aimed at examining epigenetic changes, employs polymerase chain reaction to detect immune system alterations. In addition, an increasing assortment of tools to visualize the subcellular distribution of tagged macromolecules is becoming available onboard the ISS. For instance, the NASA LMM (Light Microscopy Module) is a flexible light microscopy imaging facility that enables imaging of physical and biological microscopic phenomena in microgravity. Another light microscopy system modified for use in space to image life sciences payloads is initially used by the Heart Cells investigation ("Effects of Microgravity on Stem Cell-Derived Cardiomyocytes for Human Cardiovascular Disease Modeling and Drug Discovery"). Also, the JAXA Microscope system can perform remotely controllable light, phase-contrast, and fluorescent observations. And upcoming confocal microscopy capability will allow for optical sectioning of biological tissues to determine microanatomical localization of biomarkers. Furthermore, NASA's geneLAB effort addresses integration of genomic, epigenomic, transcriptomic, proteomic and metabolomic datasets, by applying an innovative open source science platform for multi-investigator high throughput utilization of the ISS. In sum, the expanding ISS capability for analysis of biomolecules is enabling innovative research in a broad spectrum of areas such as cellular and molecular biology, biotechnology, tissue engineering, biomedicine, and Omics, providing manifold benefits for humanity.
    Keywords: Life Sciences (General)
    Type: JSC-CN-36567 , Annual Meeting of the American Society for Gravitational and Space Research; Oct 26, 2016 - Oct 29, 2016; Cleveland, OH; United States
    Format: application/pdf
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  • 6
    Publication Date: 2019-07-18
    Description: Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LNI) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered Trademark) Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark) software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens.
    Keywords: Life Sciences (General)
    Type: 2004 ASGSB Meeting; Nov 09, 2004 - Nov 12, 2004; Brooklyn, NY; United States
    Format: text
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  • 7
    Publication Date: 2019-07-18
    Description: Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LN1) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate Containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered TradeMark)Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark)a software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens.
    Keywords: Life Sciences (General)
    Type: JSC-CN-8736 , 2004 ASGSB Meeting; Nov 09, 2004 - Nov 12, 2004; Brooklyn, NY; United States
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  • 8
    Publication Date: 2019-07-13
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: Gravitational and space biology bulletin : publication of the American Society for Gravitational and Space Biology (ISSN 1089-988X); 18; 2; 99-100
    Format: text
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