ISSN:
1570-7458
Keywords:
LepidopteraEphestia kuehniella
;
α-amylase
;
Triticum aestivum
;
endosperm
;
α-amylase inhibitors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Crude α-amylase preparations from seven Lepidoptera pests were susceptible to inhibition by salt-soluble proteins of bread wheat (Triticum aestivum L.) endosperm. Protein fractions that corresponded to tetrameric, dimeric, and monomeric wheat α-amylase inhibitors, were decreasingly effective against the insect α-amylase activity. To further confirm these results, purified inhibitors were tested against an α-amylase preparation fromEphestia kuehniella (Zeller). This preparation showed decreased activity when increasing amounts of an heterotetrameric inhibitor (reconstituted from its isolated subunits WTAI-CM2, -CM3 and -CM16) were assayed. Activity was only partially inhibited by homodimeric (WDAI-1, synonym 0.53; WDAI-2, synonym 0.19) and monomeric (WMAI-1, synonym 0.28) inhibitors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02381546
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