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  • 1
    Electronic Resource
    Electronic Resource
    Homologous recombination between plasmid DNA molecules in maize protoplasts (1991)
    Springer
    Molecular genetics and genomics 230 (1991), S. 209-218 
    Details
    ISSN: 1617-4623
    Keywords: Homologous recombination ; Protoplast transformation ; β-Glucuronidase ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The requirements for homologous recombination between plasmid DNA molecules have been studied using the PEG (polyethylene glycol)-mediated transformation system of maize (Zea mays L.) protoplasts coupled with the transient expression assay for β-glucuronidase (GUS). Two plasmids were introduced into maize protoplasts; one plasmid (pB×26) contained a genomic clone of the Adh1 maize gene; the other plasmid (piGUS) was a promoterless construction containing part of intron A of the Adhl gene fused to the gusA coding sequence. Thus, the two vectors shared an effective homologous region consisting of a 459 by (Hindlll—PvuII) fragment of the yAdh1 intron A sequence. An active gusA fusion gene would result upon homologous recombination between the plasmids within the intron A sequence, and indeed GUS activity was observed in extracts following co-transformation of maize protoplasts with the two plasmids. The presence of recombinant DNA molecules in protoplast DNA isolated 1 day after co-transformation was verified using polymerase chain reactions (PCR) and Southern blots. For efficient homologous recombination, both plasmids had to be linearized. The recombination reaction was induced by restriction of the plasmid molecules either inside the effective homologous region or at the borders of the intron sequence. However, the presence of even small, terminal, nonhomologous sequences at the 3′ end of the pB×26 fragment inhibited the recombination reaction. Also, both ends of the linearized piGUS DNA molecules were involved in the recombination reaction. The results revealed some features of homologous recombination reactions occurring in plant cells which cannot be accommodated by mechanisms postulated for similar reactions in animal system and in lower eukaryotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290670
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    Paper (German National Licenses)
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  • 2
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    Unknown
    Evaluation of thermal insulation materials (1973)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: Data was obtained on silicone-bonded fiberglass, isocyanurate foam, and two dozen other insulators. Materials were selected to withstand heat sterilization, outer space, and the Martian atmosphere. Significant environmental parameters were vibration, landing shock, and launch venting.
    Keywords: MATERIALS
    Type: NPO-11586
    Format: application/pdf
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  • 3
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    Petrology of mare-type basalt clasts from consortium breccia 73255 (1980)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Breccia 73255 contains sparse small clasts of basalts that have mare affinities; the basalts are members of a single differentiated suite. The clasts have been fractured throughout and locally granulated. Textural and compositional evidence indicates that the fracturing and granulation were produced by the 73255 breccia-forming event, and this event took place before the basalts had completely solidified. Thus, it appears that the eruption of the basalts took place at about the same time as the formation of the 73255 breccia, about 3.9 b.y. ago. The mineral assemblages and modes of the basalt clasts are those typical of mare basalts, but the clasts are not identical in detail with any basalts returned from the mare surfaces. They are instead most similar to the 14053-type basalts from the Apollo 14 site.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 17, 1980 - Mar 21, 1980; Houston, TX
    Format: text
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  • 4
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    Thermal and mechanical history of granulated norite and pyroxene anorthosite clasts in breccia 73255 (1979)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 19, 1979 - Mar 23, 1979; Houston, TX
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  • 5
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    Consortium breccia 73255 - Laser /Ar-39/-/Ar-40/ dating of aphanite samples (1979)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Laser (Ar-39)-(Ar-40) studies of clasts and groundmass in breccia 73255 are presented. They show a variety of ages indicating that some materials lost argon from sites that outgas at low temperature, while other materials have retained argon generated before the breccia-forming event. The estimate for the time of the breccia-forming event from thermal release studies is compared to the upper limit of the event from the laser studies; this work also shows that ages in excess of 4.0 G.y. determined on rocks containing devitrified maskelynite clasts probably have no chronological significance.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 19, 1979 - Mar 23, 1979; Houston, TX
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  • 6
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    Consortium breccia 73255 - Genesis and history of two coarse-grained 'norite' clasts (1979)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: A study of two clasts of coarse-grained anorthositic-noritic-troctolitic-suite norites from the fragment-laden melt rock 73255 is presented. Both clasts are igneous rocks crystallized from melts that were probably internally generated; clast 27,80 is a cumulate, and the parent magmas of both rocks probably crystallized below the lunar surface. Despite similar history, the parent rocks of the two clasts appear to be derived from distinct magma types: the 27,80 is characterized by plagioclase and orthopyroxene as the major constituents and by a high K, Nb, and Zr content, while the 27,45 type also contains augite and has a low content of K, Nb, and Zr.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 19, 1979 - Mar 23, 1979; Houston, TX
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  • 7
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    Lunar ferroan anorthosite 60025 - Petrology and chemistry of mafic lithologies (1991)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Eleven splits from the mafic-mineral-rich part of anorthosite 60025 were studied in order to establish the exact nature and causes of compositional variations in the minerals of lunar ferroan anorthosites. All splits were analyzed by INAA, and five were studied intensively by petrologic techniques. All splits were found to have similar cataclastic textures and show textural evidence of at least two episodes of deformation. The whole-rock split contains mafic minerals having a wide range of compositions and is probably polymict. It is suggested that the rare-earth patterns for all splits can be duplicated safactorily, assuming that the equilibrium liquids had flat, or nearly flat, chondrite-normalized rare-earth patterns. The plagioclases in all splits were found to be identical. Data obtained indicate that in ferroan anorthosites An content in plagioclase and mg' of associated mafic minerals are not strongly correlated.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 12, 1990 - Mar 16, 1990; Houston, TX; United States
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  • 8
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    Lunar ferroan anorthosites - Mineralogy, compositional variations, and petrogenesis (1993)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Results of a study of 16 samples from the anorthositic ferroan subgroup, or 'typical' ferroan anorthosites, are reported. The presence of heterogeneous, bimodal, and/or trimodal pyroxene compositions suggests that some of the anorthosites are polymict rocks; however, they are composed entirely of ferroan-anorthosite-suite lithologies. It is suggested that complex processes operated during the formation of the ferroan anorthosites. It is argued that original igneous compositional characteristics were altered during and/or after crystallization. Processes operative during anorthosite formation may have included some mixing of different melts, trapping of variable amounts of intercumulus liquid, postcrystallization redistribution of elements, or perturbations both during adcumulus growth and subsequent to crystallization by impact events.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Journal of Geophysical Research (ISSN 0148-0227); 98; E5; p. 9089-9105.
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  • 9
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    Comparative thermal histories of matrix from Apollo 17 Boulder 7 fragment-laden melt rocks - An analytical transmission electron microscopy study (1980)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 17, 1980 - Mar 21, 1980; Houston, TX
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  • 10
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    Granulatic breccia clasts and feldspathic melt breccia clasts from North Ray crater breccia 67975 - Precursors and petrogenesis (1989)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: A petrologic study of crystalline lithic clasts from feldspathic breccia 67975, collected on the rim of North Ray crater at the Apollo 16 site, is presented. A light gray group has been identified as granulitic breccias, and a dark gray group has been identified as feldspathic microporphyritic melt breccias. It is suggested that complete homogenization of the minerology of the granulitic breccias may have been prevented by their incorporation into the 67975 fragmental breccia, and that metamorphism of the clasts may have been interrupted by this breccia forming event.
    Keywords: LUNAR AND PLANETARY EXPLORATION
    Type: Lunar and Planetary Science Conference; Mar 14, 1988 - Mar 18, 1988; Houston, TX; United States
    Format: text
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    NASA TECHNICAL REPORTS
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