ISSN:
1432-0983
Keywords:
Keywords RNA editing
;
mRNA heterogeneity
;
Polymorphisms
;
C to U editing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract C to U editing of apt9, nad3, and cox2 mRNAs was investigated in maize seedlings at various developmental stages as well as in suspension-cultured cells. Heterogeneity of mRNAs that result from incomplete editing was analyzed for each gene and from five tissues or developmental conditions. The editing status of approximately 30 cDNA clones was determined by digestion with a restriction enzyme that discriminates between unedited and edited DNA sequences. The atp9 and spliced cox2 cDNAs were essentially completely edited in all samples examined. Analysis of three editing sites of nad3 cDNAs indicated that incompletely edited cDNAs were detected in all tissues and treatments with a temporal increase in the overall editing status, from 50% at 3 days to about 75% at 7 days. These results indicate that incompletely edited mRNAs are prevalent for some plant mitochondrial genes, and can change with developmental or growth conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050085
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