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  • Keywords: nitrile hydratase; thermophile; Bacillus smithii  (1)
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    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 20 (1998), S. 220-226 
    ISSN: 1476-5535
    Keywords: Keywords: nitrile hydratase; thermophile; Bacillus smithii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A novel thermophilic Bacillus smithii strain SC-J05-1, isolated from a hot spring, had the ability of hydrating nitrile to form amide. The nitrile hydratase was purified to homogeneity from the microbial cells of SC-J05-1 and was characterized. The enzyme was a 130-kDa protein composed of two different subunits (25.3 kDa and 26.8 kDa) and contained cobalt ions. This enzyme had the optimal temperature of 40°C and was stable up to 50°C. The optimal pH was in the alkaline region higher than pH 10.
    Type of Medium: Electronic Resource
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