ISSN:
1476-5535
Keywords:
Keywords: diatom; Nitzschia laevis; EPA; glucose; silicate; heterotrophic
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The effects of silicate and glucose on growth and eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis were studied. By alternately altering the concentrations of silicate (2.7–64 mg l−1) and glucose (1–40 g l−1) in the medium, the highest cell dry weight (ca. 5.5 g l−1) was obtained at 20 g l−1 glucose and 32 mg l−1 silicate, while the highest specific growth rate (ca. 0.65 day−1) was obtained at a relatively low glucose concentration (5 g l−1) and high silicate concentrations (32–64 mg l−1). At glucose levels of 5 and 20 g l−1, EPA content was higher with lower silicate concentrations (2.7 and 16 mg l−1 silicate, respectively), while at a silicate level of 16 mg l−1, higher glucose concentrations (20–40 g l−1) facilitated EPA formation. The highest EPA yield (131 mg l−1) was obtained at 20 g l−1 glucose and 32 mg l−1 silicate, while the highest EPA productivity (15.1 mg l−1 day−1) was obtained at 20 g l−1 glucose and 64 mg l−1 silicate. Journal of Industrial Microbiology & Biotechnology (2000) 25, 218–224.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/sj.jim.7000056
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