ISSN:
1432-203X
Keywords:
Key wordsCatharanthus roseus
;
Differentiation
;
Hairy roots
;
Tryptophan decarboxylase
;
Strictosidine synthase
;
3-hydroxymethylglutaryl coenzyme A reductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In vitro cultures of hairy root derived from Catharanthus roseus accumulate higher levels of indole alkaloids than cell suspension cultures. Hairy roots were interconverted to undifferentiated cells by manipulation of the culture medium. When the concentration of micronutrients in the culture medium was five times that of Phillips and Collins (1979) medium, cell suspensions formed from the hairy roots. The alkaloid content was five times lower in the cell suspensions than in the control, but upon regeneration of the roots the alkaloid content regained its original level. The formation of cell suspensions from hairy roots was also accompanied by a reduction in tryptophan decarboxylase and the strictosidine synthase activity to less than 5% and 30%, respectively. 3-Hydroxymethylglutaryl coenzyme A reductase activity was the same in the cell suspension and in the regenerated line.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050539
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