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  • 1
    Electronic Resource
    Electronic Resource
    Leaf anatomy and ultrastructure of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana (1988)
    Springer
    Planta 173 (1988), S. 183-189 
    Details
    ISSN: 1432-2048
    Keywords: Crassulacean acid metabolism (leaf structure) ; Cytosolic vesicles ; Kalanchoe ; Leaf ultrastructure and venation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-microscopic analysis of leaf clearings of the obligate Crassulacean-acid-metabolism (CAM) species Kalanchoe daigremontiana Hamet et Perr. has shown the existence of unusual and highly irregular venation patterns. Fifth-order veins exhibit a three-dimensional random orientation with respect to the mesophyll. Minor veins were often observed crossing over or under each other and over and under major veins in the mesophyll. Paraffin sections of mature leaves show tannin cells scattered throughout the mesophyll rather evenly spaced, and a distinct layer of tannin cells below the abaxial epidermis. Scanning electron microscopy showed that bundle-sheath cells are distinct from the surrounding mesophyll in veins of all orders. Transmission electron microscopy demonstrated developing sieve-tube elements in expanded leaves. Cytosolic vesicles produced by dictyosomes undergo a diurnal variation in number and were often observed in association with the chloroplasts. These vesicles are an interesting feature of cell ultrastructure of CAM cells and may serve a regulatory role in the diurnal malic-acid fluctuations in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403009
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  • 2
    Electronic Resource
    Electronic Resource
    Plasmalemma- and tonoplast-ATPase activity in mesophyll protoplasts, vacuoles and microsomes of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana (1988)
    Springer
    Planta 173 (1988), S. 190-196 
    Details
    ISSN: 1432-2048
    Keywords: ATPase ; Crassulacean acid metabolism (ATPase activity) ; Kalanchoe ; Plasmalemma ; Tonoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adenosine-triphosphatase activity on the plasmalemma and tonoplast of isolated mesophyll protoplasts, isolated vacuoles and tonoplast-derived microsomes of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana Hamet et Perr., was localized by a cytochemical procedure using lead citrate. Enzyme activity was detected on the cytoplasmic surfaces of the plasmalemma and tonoplast. The identity of the enzymes was confirmed by various treatments differentiating the enzymes by their sensitivity to inhibitors of plasmalemma and tonoplast H+-ATPase. Isolated vacuoles and microsomes prepared from isolated vacuoles clearly exhibited single-sided deposition on membrane surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403010
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    Paper (German National Licenses)
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