ISSN:
1573-5044
Keywords:
Ipomoea
;
morning glory
;
cell culture
;
acid phosphatases
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two acid phosphatases isolated from culturedIpomoea (moring glory) cells were separated by column chromatography on DEAE-cellulose. The two acid phosphatases have different pH optima (pH 4.8–5.0 and 6.0) and do not require the presence of divalent ions. The enzymes possess high activity toward pyrophosphate,p-nitrophenylphosphate, nucleoside di- and triphosphates, and much less activity toward nucleoside monophosphates and sugar esters. The two phosphatases differ from each other in Michaelis constants, in the degree of inhibition by arsenate, fluoride and phosphate and have quantitative differences of substrate specificity. In addition, they also differ in their response to various ions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02318923
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