ISSN:
1617-4623
Keywords:
Bacillus thuringiensis var. israelensis
;
Mosquito larvicidal δ-endotoxin
;
T7 RNA polymerase
;
cytA gene product
;
Inhibition of DNA synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal δ-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene 1, under the control of lacP. Loss of colony-forming ability without substantial lysis, associated with immediate inhibition of DNA synthesis, was observed after induction of transformed cells. The cytA gene product may kill E. colicells by disrupting their chromosome replicating apparatus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00299149
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