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  • 1
    Electronic Resource
    Electronic Resource
    Inheritance and mapping of isoenzymes in pea (Pisum sativum L.) (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 559-566 
    Details
    ISSN: 1432-2242
    Keywords: Inheritance ; Linkage ; Gene mapping ; Isoenzymes ; Pisum sativum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three isoenzyme systems (amylase, esterase and glutamate oxaloacetate transaminase) were examined in seeds of pea (Pisum sativum L.) and shown to give clear variation in their band patterns on gel electrophoresis between different lines. The inheritance of these isoenzyme systems, and the location of their genes on the pea genome was investigated. Reciprocal crosses were made between lines, F2 seeds were analysed for segregation in the band patterns of the isoenzymes, and F2 plants were investigated to find linkage between the genes for these isoenzymes and genes for selected morphological markers. The results obtained showed that each of the investigated isoenzyme systems is genetically controlled by co-dominant alleles at a single locus. The gene for amylase was found to be on chromosome 2, linked to the loci k and wb (wb ... 9 ... k ... 25 ... Amy). The gene for esterase was found to be linked with the gene Br (chromosome 4) but the exact location is uncertain because of the lack of the morphological markers involved in the cross. The gene for glutamate oxaloacetate transaminase was found to be on chromosome 1 and linked with the loci a and d (a... 24... Got... 41 ... d).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285014
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in polypeptides of the major albumin protein of pea (Pisum sativum L.) : Inheritance and molecular analysis (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 277-281 
    Details
    ISSN: 1617-4623
    Keywords: Pea albumins ; Inheritance ; Molecular analysis ; Null mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The variation in the polypeptides of the major albumin (MA) protein of different pea genotypes was studied. The two MA polypeptides, MA-L and MA-S, behave as products of single Mendelian genes at a single locus. The band patterns of fragments of restricted genomic DNA of four pea genotypes, detected by hybridisation with a cDNA encoding MA, revealed differences in the number and sizes of DNA fragments related to MA. This is discussed in relation to MA protein structure. The relative amount of MA protein in seeds appears to be strongly influenced by the total number of gene copies present in a genotype. Through molecular analysis at the protein, RNA and genomic DNA levels, a mutant line was identified which carries a deletion of the active MA structural genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261188
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