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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 98 (1999), S. 496-501 
    ISSN: 1432-2242
    Keywords: Key words Helianthus annuus ; Sunflower mutant ; Palmitic acid ; Inheritance ; Fatty acid composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sunflower genotypes with increased levels of palmitic acid (C16 : 0) in the seed oil could be useful for food and industrial applications. The objective of the present study was to determine the inheritance of the high C16 : 0 content in the sunflower mutant line CAS-5 (〉25% of the total oil fatty acids). This mutant was reciprocally crossed with the lines HA-89 (5.7% C16 : 0) and BSD-2-691 (5.4% C16 : 0), the latter being the parental line from which CAS-5 was isolated. No maternal effect for the C16 : 0 content was observed from the analysis of F1 seeds in any of the crosses. The inheritance study of the C16 : 0 content in F1, F2 and BC1F1 seeds from the crosses of CAS-5 with its parental line BSD-2-691 indicated that the segregation fitted a model of two alleles at one locus with partial dominance for the low content. The analysis of the fatty acid composition in the F2 populations from the crosses with HA-89 revealed a segregation fitting a ratio 19 : 38 : 7 for low (〈7.5%), middle (7.5–15%), and high (〉25%) C16 : 0 content, respectively. This segregation was explained on the basis of three loci (P1, P2, P3) each having two alleles showing partial dominance for low content. The genotypes with a high C16 : 0 content were homozygous for the recessive allele p1 and for at least one of the other two recessive alleles, p2 or p3. This model was further confirmed with the analysis of the F3 and the BC1F1 generations. It was concluded that both the recessive alleles p2 and p3 were already present in the BSD-2-691 line, the allele p1 being the result of a mutation from P1. This genetic study will facilitate breeding strategies associated with the incorporation of the high C16 : 0 trait into agronomically acceptable sunflower hybrids.
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Sunflower ; Helianthus annuus ; High palmitic acid ; High stearic acid ; Epistatic interaction ; Inheritance ; Oil quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Two sunflower (Helianthus annuus L.) mutants with high concentrations of saturated fatty acids in their seed oil have been identified and studied extensively. The mutant line CAS-5 has high concentrations of palmitic acid (C16:0) (〉25% compared with 7% in standard sunflower seed oil) and low-C18:0 values (3%). CAS-3 is characterized by its high levels of stearic acid (C18:0) (〉22% compared with 4% in standard sunflower seed oil) and a low-C16:0 content (5%). CAS-5 also possesses elevated levels of palmitoleic acid (C16:1) (〉5%), which is absent in standard sunflower seed oil. The objective of this study was to determine the relationships between the loci controlling the high-C16:0 and the high-C18:0 traits in these mutants. Plants of both mutants were reciprocally crossed. Gas chromatographic analyses of fatty acids from the seed oil of F1, F2, F3 and the BC1F1 to CAS-5 generations indicated that the loci controlling the high-C16:0 trait exerted an epistatic effect over the loci responsible for the high-C18:0 character. As a result, the phenotypic combination containing both the high-C16:0 levels of CAS-5 and the high-C18:0 levels of CAS-3 was not possible. However, phenotypes with a saturated fatty acid content of 44% (34.5% C16:0+9.5% C18:0) were identified in the F3 generation. These are the highest saturated (C16:0 and C18:0) levels reported so far in sunflower seed oil. When F3 C16:0 segregating generations in both a high- and a low-C18:0 background were compared, the high-C16:1 levels were not expressed as expected in the high-C18:0 background (CAS-3 background). In this case, the C16:1 content decreased to values below 1.5%, compared with 〉5% in a low-C18:0 background. As the stearoyl-ACP desaturase has been reported to catalyze the desaturation from C16:0-ACP to C16:1-ACP, these results suggested that a decrease in its activity was involved in the accumulation of C18:0 in the high-C18:0 mutant CAS-3.
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  • 3
    ISSN: 1573-0417
    Keywords: saline lakes ; stable isotope ; sedimentology ; mineralogy ; paleohydrology ; Altiplano ; Holocene ; Little Ice Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The paleohydrological evolution of several high altitude, saline lakes located in the southernmost Altiplano (El Peinado and San Francisco basins, Catamarca province, NW Argentina) was reconstructed applying sedimentological, geochemical and isotopic techniques. Several playa lakes from the San Francisco basin (26° 56′ S; 68° 08′ W, 3800-3900 m a.s.l.) show evidence of a recent raise in the watertable that led to modern deposition of carbonate and diatomaceous muds. A 2 m - long core from El Peinado Lake (26° 29′ 59′′ S, 68°05′ 32′′ W, 3820 m a.s.l.) consists of calcitic crusts (unit 3), overlaid by an alternation of macrophyte-rich and travertine clast- rich, laminated muds (unit 2), and topped by travertine facies (unit 1). This sedimentary sequence illustrates a paleohydrological evolution from a subaerial exposure (unit 3) to a high lake stand (unit 2), and a subsequent smaller decrease in lake level (unit 1). The δ13Corganic matterrecord also reflects the lake transgression between units 3 and 2. Although there is a general positive correlation between δ 18Ocarbonate and salinity proxies (Na, Li and B content), the large data dispersion indicates that other factors besides evaporation effects control chemical and isotopic composition of lakewater. Consequently, the oxygen isotopic composition cannot be interpreted exclusively as an indicator of salinity or evaporation ratio. The degassing of CO2 during groundwater discharge can explain the enriched δ13C values for primary carbonates precipitated. The carbon budget in these high altitude, saline lakes seems to be controlled by physical rather than biological processes.The Altiplano saline lakes contain records of environmental and climatic change, although accurate 14C dating of these lacustrine sediments is hindered by the scarcity of terrestrial organic material, and the large reservoir effects. Sedimentologic evidence, a 210Pb-based chronology, and a preliminary U/Th chronology indicate a very large reservoir effect in El Peinado, likely as a result of old groundwaters and large contributions of volcanic and geothermal 14C-free CO2 to the lake system. Alternative chronologies are needed to place these paleorecords in a reliable chronological framework. A period of increased water balance in the San Francisco basin ended at about 1660 ± 82 yr B.P. (calendar yr U/Th age), and would correlates with the humid phase between 3000 and 1800 yr B.P detected in other sites of the southern Altiplano. Both, 210Pb and preliminary U/Th dating favor a younger age for the paleohydrological changes in El Peinado. The arid period reflected by subaerial exposure and low lake levels in unit 3 would have ended with a large increase in effective moisture during the late 17th century. The increased lake level during deposition of unit 2 would represent the period between AD1650 - 1900, synchronous to the Little Ice Age. This chronological framework is coherent with other regional records that show an abrupt transition from more arid to more humid conditions in the early 17th century, and a change to modern conditions in the late 19th century. Although there are local differences, the Little Ice Age stands as a significant climatic event in the Andean Altiplano.
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  • 4
    ISSN: 1573-0417
    Keywords: sedimentology ; saline lake ; meromixis ; Northern Great Plains ; Holocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Medicine lake is a small (about 1 km2), shallow (up to 10 m deep), saline (50–170 g l−1) and meromictic lake formed after the retreat of the Wisconsin ice in the north American Great Plains. Based on a detailed sedimentological analysis of cores, we describe and interpret 13 sedimentary subfacies grouped in 9 associations which characterize the following lacustrine subenvironments: clastic littoral (freshwater and saline), springs, microbial mats, bench slope, and pelagial (oxic, alternating oxic-anoxic, anoxic and hypersaline, and organic-dominated). Lateral distribution and vertical evolution of subfacies in our model are controlled by climate fluctuations, climate-related limnological parameters (lake level, TDS and brine composition, and redox conditions), and autocyclic processes (progressive infilling of the basin and higher sedimentation rate in the pclagial realm). Microbial and chemical processes govern deposition in this system, and meromixis plays a decisive role in lake dynamics. Phototropic bacterial plate communites at the chemocline dominated as pelagial organic producers during stable meromictic periods, whereas benthic microbial communities developed during mixed water periods. Water stratification during the Holocene was mainly controlled by three parameters: 1) basin morphometry, 2) lake level, and 3) differences in TDS values between mixolimnion and monimolimnion waters. Sedimentary facies analyses is a powerful descriptive and interpretative tool that greatly contributes to deciphering the high resolution paleoenvironmental information archived in lake sequences. Depositional and paleoenvironmental models provide a dynamic framework for integrating paleolimnological data and other proxy paleorecords. Medicine lake serves as a facies model for shallow, perennial hypersaline, meromictic lakes in modern and ancient lacustrine basins. The sediment sequence from Medicine lake cores is consistent with the general paleoclimatic evolution of the northern Great Plains since the retreat of ice sheets. Our study reveals a plethora of rapid fluctuations in the water cycle both during the middle and the late Holocene. These augment prior paleoclimate reconstructions based on diatom studies of the lower Holocene freshwater to saline transition and on pollen profiles which show little variability during the subsequent long prairie grass episode.
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  • 5
    ISSN: 1573-0417
    Keywords: Atacama ; Holocene ; limnogeology ; South America ; paleoclimatology ; lake sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Sedimentological, mineralogical and geochemical analyses of sediment cores from 9 m-deep, saline Laguna Miscanti, Chile (23 ° 44′S, 67 °46′W, 4140 m a.s.l.) together with high-resolution seismic profiles provide a mid to late Holocene time series of regional environmental change in the Atacama Altiplano constrained by 210Pb and conventional 14C dating. The mid Holocene was the most arid interval since the last glacial maximum, as documented by subaerial exposure and formation of hardgrounds on a playa surface. Extremely low lake levels during the mid Holocene appear consistent with lower effective moisture recorded at other sites along the Altiplano and in the Amazon Basin. Termination of this arid period represented a major shift in the regional environmental dynamics and inaugurated modern atmospheric conditions. The cores show a progressive upward increase in effective moisture interrupted by numerous century-scale drier periods of various intensities and durations that characterize a fluctuating late Holocene climate. In spite of chronological uncertainties, the major environmental changes seem to correlate with the available paleorecords from the region providing a coherent account of effective moisture variability in the tropical highlands of South America.
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