ISSN:
1432-1432
Keywords:
Structure-based scoring matrix MDPRE
;
Phyletic relationship of protein structure
;
Spatial preference factor
;
Sequence alignment
;
Protein homology detect
;
Globins
;
Immunoglobulins
;
Cytochromes c
;
Serine proteinases
;
Dinucleotide-binding domains
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A structure-based scoring matrix MDPRE was derived from amino acid spatial preferences in protein structures. Sequence alignment and evolutionary studies by using MDPRE matrix gave similar results as those from ordinary sequence and structure alignments. It is interesting that a matrix derived from structure data solely could give comparable alignment results, strongly indicating the intimate connection between protein sequences and structures. The branch order and length from this approach were close to those obtained by a structure comparison method. Thus, by applying this structure-based matrix, the trees obtained should reflect evolutionary characteristics of protein structure. This approach takes advantage over a direct structure comparison in that (1) only a sequence and MDPRE matrix are needed, making it simple and widely applicable (especially in the absence of 3-dimensional protein structure data); (2) an established algorithm for sequence alignment and tree building could be employed, providing opportunities for direct comparison between matrices from different methodologies. One of the most striking features of this method is its capability to detect protein structure homologies when the sequence identities are low. This was well reflected in the given examples of the alignment of dinucleotidebinding domains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02407306
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