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  • Chemistry  (195)
  • Immunofluorescence  (4)
  • Atomic, Molecular and Optical Physics
  • Magnetism
  • 1975-1979  (50)
  • 1965-1969  (150)
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Keywords
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 279-286 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin ; Immunofluorescence ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibodies were raised in rabbits against 2Fe−2S ferredoxin from N. tabacum L. The antibodies showed partial cross-reactivity in the double diffusion test with ferredoxins from Spinacia oleracea L., Petunia inflata Fries., P. axillaris Lam., Phaseolus vulgaris L., Chlamydomonas remhardii Dang. A complete cross-reaction was observed with ferredoxins from five other Nicotiana species, thus with this test it was impossible to discriminate between these ferredoxins. Therefore the following test was performed. Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Rabbit anti-N. tabacum-serum was then pre-incubated with this ferredoxin which resulted in complete abolition of cross-reactivity with free heterologous ferredoxin. However, the serum retained antibody activity against specific antigenic determinants of N. tabacum ferredoxin. When this serum was tested against ferredoxin purified from the hybrid: N. tabacum (♀)xN. glutinosa (♂) it gave a positive reaction. The relative content of maternal N. tabacum ferredoxin in the hybrid was estimated by using a fluorescent derivative of this specific antibody and estimating the cross-reactivity compared with that of artificial mixtures of pure N. tabacum and N. glutinosa ferredoxins. The hybrid contained 50% of maternal ferredoxin. This technique was also applied to ferredoxins of other species of Nicotiana and to the ferredoxin from the hybrid N. clevelandii (♀)xN. glutinosa (♂). We conclude that it provides a good test system for the study of the expression of chloroplast ferredoxin in Nicotiana hybrids in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388163
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  • 2
    Electronic Resource
    Electronic Resource
    The gonadotropic cells in the pituitary of the black molly, Mollienisia latipinna, and other teleosts, identified by the immunofluorescence technique in normal and androgen-treated animals (1976)
    Springer
    Cell & tissue research 167 (1976), S. 211-219 
    Details
    ISSN: 1432-0878
    Keywords: Teleosts ; Pituitary ; GTH ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using anti-carp-gonadotropic-γ-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong fluorescence was observed in the ventralmost region, containing the presumed gonadotropic cells; weak fluorescence was observed in the dorsal region, in the presumed thyrotropic cells. The possibility of an unspecific reaction with TSH-cells in the latter region is discussed. Treatment of male black mollies for 38 days with methyltestosterone resulted in a loss of fluorescence in the ventral region of the meso-adenohypophysis, whereas there was no decrease of the fluorescence in the dorsal region. The results support the hypothesis that the ventral basophils in the mesoadenohypophysis produce gonadotropic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224328
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  • 3
    Electronic Resource
    Electronic Resource
    Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the fore-brain and the neurohypophysis of the green frog, Rana esculenta L. (1976)
    Springer
    Cell & tissue research 168 (1976), S. 325-333 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropin Releasing Hormone ; Fore-brain ; Rana esculenta ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using antibodies against mammalian LH-RH, the double antibody immunofluorescence technique has been applied to serial cross sections of the brains of adult Rana esculenta. Immunoreactive material was found in perikarya of an unpaired nucleus in front of the preoptic recess. The axons of these perikarya also contain fluorescing material. They form a single bundle which passes under the preoptic recess, than splits into two tracts, one on either side of the optic chiasm. The two tracts reunite just before entering the median eminence. The axons end near the capillaries in the outer zone of the median eminence. The possibility of two separate centres for the stimulation of gonadotropic activity in the brains of anurans is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215310
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  • 4
    Electronic Resource
    Electronic Resource
    Calorimetric investigation of the influence of polymer concentration on the transition enthalpy in the helix-coil transition of the double-stranded helical complex poly(A + U) (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 611-613 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360070416
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  • 5
    Electronic Resource
    Electronic Resource
    Determination of the base-pairing content of four specific tRNAs by infrared spectroscopy (1977)
    New York : Wiley-Blackwell
    Biopolymers 16 (1977), S. 1735-1745 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Infrared spectroscopy was used for the determination of the base-pairing content of four specific tRNAs in deuterium oxide solution. Infrared spectra were obtained in the 1750-1550 cm-1 region at various temperatures ranging from about 15 to 90°C. Melting curves were constructed by plotting the molar extinction coefficient at ν = 1657 cm-1 versus temperature. These transition curves enabled us to determine the ranges of temperature which correspond to the ordered (partially double-stranded) or randomly coiled structure of the tRNA. For a set of wavenumbers the extinction coefficients at these temperatures were used for the calculation of the base-pairing content. The procedure employed here is based on a method described earlier by Thomas [(1969) Biopolymers 7, 325-334]. For the conditions selected for this investigation (Mg2+-free D2O-buffer; 0.01M tris-DCl, 0.015M NaCl, pD 7.5) the results of this determination agree within the limits of errors with the number of base pairs predicted by the cloverleaf model.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1977.360160810
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  • 6
    Electronic Resource
    Electronic Resource
    Experimental thermodynamics of the helix-random coil transition. II. Calorimetric measurement of transition enthalpies of poly A in acid aqueous solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 423-431 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The enthalpy change accompanying the double helix-coil transition of polyriboadenylic acid (poly A) in aqueous solution has been measured optically and calorimetrically in the pH range 5.7-4.5. The course of this cooperative transition was followed optically by measuring changes in ultraviolet absorption as a function of temperature at different pH values, and calorimetrically by determining the heat capacity of the solution through the transition region. From the latter measurements, the enthalpy of transition was calculated. It is shown, that ΔH is dependent on pH as it is expected from the influence of protonation of the double helix of poly A.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360070313
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  • 7
    Electronic Resource
    Electronic Resource
    Notiz über die Bildung von Benzyloxycarbonyl-α-aminoglutarimid aus Benzyloxycarbonyl-L-glutamin (1969)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 102 (1969), S. 2158-2159 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19691020645
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  • 8
    Electronic Resource
    Electronic Resource
    PCILO calculations on hydrogen bonded complexes. Dimers involving second row atoms (1977)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 12 (1977), S. 805-811 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The PCILO method was used to study systems with the following types of intermolecular hydrogen bonds, involving 2nd row atoms: N…H—S, S…H—S, N…H—Cl, Cl…H—F, Cl…H—Cl, P…H—N, P…H—P, P…H—F, S…H—F.In all the cases calculated, the PCILO method yields an accurate qualitative description of the stabilization energies and to some extent quantitatively good agreement with reliable ab initio and experimental results. The corresponding equilibrium distances are in almost all cases smaller (up to 0.6 Å) than the ab initio values of the same compounds.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560120504
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  • 9
    Electronic Resource
    Electronic Resource
    Free radicals in colloidal systems: Formation and decay of radicals in the tensides α-hexadecyl-ω-hydroxypoly(oxyethylene) and α-tetradecyl-ω-(sodium sulfonato)tri(oxyethylene) (1978)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 179 (1978), S. 2279-2295 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Organic radicals were produced in the tensides C16H33(OCH2CH2)21OH and C14H29(OCH2CH2)3-SO3Na in aqueous solutions using a short pulse of high energy electrons. The radicals were formed by OH attack on the (OCH2CH2)x-parts of the tensides. The decay of the 250nm absorption of the radicals was recorded at different initial radical concentrations and tenside concentrations. Several radicals could be produced in one micelle. Radicals formed in the same micelle decay within microseconds or faster. The half life time τ1 in a micelle carrying two radicals is 2,0·10-6 s for C16H33(OCH2CH2)21 OH and less than 6·10-7 s for C14H29(OCH2CH2)3SO3Na. A model for intramicellar radical-radical reactions is proposed according to which the rate is faster in tensides of high critical micelle concentration. Single radicals in micelles of C16H33(OCH2CH2)21OH can deactivate each other without leaving the micelles. This intermicellar reaction is discussed in terms of the rate of diffusion-controlled micelle-micelle encounters, an encounter time of 7·10-8s, and the above time τ1 for intramicellar reaction. The observed rate constant 2k of intermicellar reaction is 3,5·106 mol-1·l·s-1. At low tenside concentrations, the bimolecular rate constant increases since more single tenside radicals are present in solution. They react rapidly (ca. 108 mol-1·l·s-1) with radicals in micelles. Single radicals in C14H33(OCH2CH2)3-SO3Na micelles cannot directly react with each other because of the Coulombic repulsion between two anionic micelles. Reaction occurs after the exit of a tenside radical from its micelle, the rate of which depends on the micellar equilibrium Mn⇄Mn-1+M (M: tenside molecule; n: agglomeration number of micelle). A single radical in solution reacts with a single radical in a micelle with 2k = 1,0·108 mol-1·l·s-1 and with another single radical in solution with 4,0·107 mol-1·l·s-1.γ-Irradiation of both tensides in aqueous solution leads to slight increases in viscosity, followed by turbidity beyond the “gel dose” and phase separation. These effects are explained in terms of crosslinking of tenside molecules. Formation of a large network requires bridges between all participating tenside molecules (not only bridges between micelles).
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1978.021790915
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  • 10
    Electronic Resource
    Electronic Resource
    Localisation of gonadotropin releasing hormone (GRH) in the forebrain and neurohypophysis of the trout (Salmo gairdneri) (1977)
    Springer
    Cell & tissue research 181 (1977), S. 163-168 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropin releasing hormone ; Forebrain ; Salmo gairdneri ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The double antibody immunofluorescence technique was applied to serial coronal and sagittal sections of the brains of the trout, Salmo gairdneri, using an antibody against mammalian LH-RH. Immunoreactive material was found in small perikarya, situated at both sides of the ventriculus communis in the area dorsalis pars medialis of the telencephalon. The axons of these perikarya, also containing immunoreactive material, do not form a tract, but run diffusely in a caudo-ventral direction towards the pituitary stalk. The ending of these fibres has not yet been established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219978
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