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  • Chemistry  (11)
  • Immunocytochemistry  (2)
  • 1980-1984  (10)
  • 1925-1929  (3)
  • 1
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental investigation of an impinging water jet freezing on a melting solid surface has been carried out. Attention was focused on the stagnation region of an axisymmetric jet. In the experiment, a water jet was directed upward against the lower end of a meltable rod, having a diameter about twice that of the jet orifice. Solid octane (m.p. - 56.5°C) and solid mercury (m.p. - 38.9°C) served as the meltable materials. A laminar-axisymmetric flow model was developed to describe melting heat transfer in the presence of jet solidification within the impingement region. Measurements of the melting rate and conditions for the onset of jet solidification were found to agree quite well with the values predicted with this model.
    Additional Material: 8 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 952-959 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Effects of molecular size and configuration on hindered diffusion were examined by measuring diffusion of two series of uncharged macromolecules through track-etch membranes with uniform and well-defined pore geometry. At any given Stokes-Einstein radius, diffusivities in small pores were lower for ficoll (crosslinked structure) than for dextran (more nearly linear structure).
    Additional Material: 5 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 233 (1983), S. 99-111 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Vasopression ; Osmotic stress ; RIA ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A correlative radioimmunoassay (RIA) and immunocytochemical (ICC) study was carried out on vasopressin (VP) distribution and content in brains of normal and 3-day water-deprived rats. By RIA there were statistically significant differences in brain VP per pg/mg between normal and osmotically stressed specimens in hypothalamus (338.4 versus 134.4), thalamus (4.8 versus 0.9), septum (18.0 versus 3.4), striatum (1.6 versus 0.7) and amygdala (17.3 versus 1.3), but not in other brain regions measured. Pituitary VP decreased from 71.1 to 8.7 ng/mg, and plasma VP rose from 3.6 to 19.3 pg/ml during water deprivation. Application of the peroxidase-anti-peroxidase ICC method of Sternberger to vibratome sections showed that VP-immunoreactivity in dehydrated specimens decreased in perikarya of paraventricular nucleus and suprachiasmatic nucleus, while intrahypothalamic immunoreactive magnocellular fibers appeared more conspicuous due to proliferation of large Herring bodies. In extrahypothalamic sites VP-immunoreactivity in water-deprived rats was visibly reduced in periventricular thalamus and septum. Thus it is apparent that both intra- and extrahypothalamic VP are affected by osmotic stress, and these results are discussed within the context of current ideas relating to co-activation of neurosecretory cells that project to different sites.
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  • 4
    ISSN: 1432-0878
    Keywords: Newborn rat epidermis ; Soluble epidermal protein ; Thiolproteinase inhibitor ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Subcellular distribution of a thiol-proteinase inhibitor protein was determined in the epidermis of the newborn rat by light and electron microscopy. This protein was highly soluble in basal cells and concentrated on ribosomes in the perinuclear region. Solubility in Tris buffer decreased in granular and cornified cells in which the protein appeared on polysomes which were attached on other cellular structures such as dense homogenous deposits and tonofilaments. The protein also appeared to be deposited on the plasma membrane and became insoluble in Tris buffer at 37° C, but solubilized in 1 M phosphate buffer. Location of the protein around keratohyalin granules or by the plasma membrane suggested that the inhibitor protein bound to cysteinerich protein of the epidermis with or without forming a thiol-proteinase inhibitor complex. The thiol-proteinase inhibitor protein seems to contribute to epidermal cell differentiation at multiple points through changes in its solubility and subcellular localization from basal cells to cornified cells.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 26 (1981), S. 1049-1056 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 10 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 26 (1981), S. 395-409 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A process has been developed by which very high-modulus semicrystalline polymer films can be extruded continuously from a melt. This is accomplished by controlled cooling of the melt in a two-stage flow channel. A temperature gradient along the flow channel quenches the melt prior to an area reduction in which the polymer undergoes solid-state orientation. Analysis of high-density polyethylene tapes extruded by this process shows that they have properties similar to samples hydrostatically extruded at 120°C. Infrared analysis was used to determine both the degree of crystallinity and degree of orientation in these tapes as well as previously prepared hydrostatically extruded samples.
    Additional Material: 9 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Biopolymers 23 (1984), S. 1249-1259 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We consider the irreversible dissociation kinetics of proteins that bind cooperatively and nonspecifically to DNA. Our model consists of an infinitely long one-dimensional nucleic acid lattice on which are bound protein ligands. A set of adjacent bound proteins forms a cluster of length n. A protein molecule may dissociate from any site within the bound cluster, not only from the ends, as was assumed in a previous model of this process due to Lohman [(1983) Biopolymers 22, 1697-1713]. By considering this additional pathway, we present a more general treatment of the dissociation kinetics of cooperatively bound ligands. We show that dissociation from the (n-2) internal positions of an n-cluster is an important pathway when the initial fractional saturation of the lattice is close to unity and the co operatively is low. When the fractional saturation is initially equal to 1 and the co operatively is low, our model does not give the zero-order dissociation kinetics predicted by the Lohman model.
    Additional Material: 3 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Biopolymers 20 (1981), S. 1651-1669 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Approximate methods are developed and evaluated for treating the rate of binding ligands that cover several contiguous sites to a homogeneous one-dimensional lattice, which represents a nucleic acid or other linear biopolymer. The model requires as input only the number of lattice sites necessary for binding, the total number (possibly infinite) of lattice sites, and elementary rate constants for the cooperative and noncooperative association and dissociation of the ligand on the lattice. The computational methods employed are an extension of the triplet closure approximation from the helix-coil (single-site ligand) problem to the large ligand binding problem. It is found that consideration of clusters of n + 2 lattice sites, where each ligand covers n sites, gives a surprisingly accurate description of the kinetics. The approximation is implemented by an extension of the matrix-iteration approach proposed by Craig and Crothers. The effects of the finite lattice length, as well as the capability to treat ligand motion along the lattice, are incorporated. When all symmetries are taken into consideration, the time required for the matrix iteration calculation rises only linearly with the ligand length n and is considerably less than that of the Monte Carlo method, which is used as a standard for comparison.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 22 (1982), S. 549-555 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Slow crack growth behavior of medium-density polyethylene pipe samples has been investigated by applying standard and nonstandard fracture mechanics test methods. Slow crack growth rate varied by several orders of magnitude among the PE2306 materials from different manufacturers. Slow crack generated surfaces exhibit fibrous textures of varying degrees of coarseness when examined under the scanning electron microscope. When the measured fiber heights, δ/2, of these samples were plotted against the applied stress intensity factor, KI, they showed a simple power law relationship (of exponent 2). Assuming that the pulled-out fibers transmit almost uniform tensile stresses across the crazed zone at the crack front, the Dugdale-Barenblatt model was introduced to predict the fiber height. Although the model needs further modification, it provides a general power law relationship between the fiber height and the applied stress intensity factor.
    Additional Material: 10 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 22 (1982), S. 27-33 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Solid state rolling of semi-crystalline polymers is shown to be an effective method of producing high strength, high modulus tape at acceptable production rates. High density polyethylene tape was produced having a tensile strength exceeding 300 MPa and a tensile modulus of 8.7 GPa at production rates exceeding 8 m/min. A significant factor in producing highly oriented tape by the rolling process is roll temperature. Increasing the roll temperature from 25°C to 125°C not only increases the maximum extent of orientation achievable, but increases the mechanical properties at a given degree of thickness reduction. Internal frictional heat development limited the maximum thickness reduction ratio of polypropylene to 6.6:1. This reduction was reached by rolling at 150°C. The resultant tape had a tensile modulus of 5.1 GPa and a tensile strength of 300 MPa.
    Additional Material: 8 Ill.
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