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Digitale Medien

Calmodulin-like activity in a mineralising tissue: The rat molar tooth germ (1981)

Hubbard, Michael J. ; Bradley, Mark P. ; Kardos, Thomas B. ; [weitere]

Springer

Calcified tissue international 33 (1981), S. 545-548

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ISSN: 1432-0827

Schlagwort(e): calmodulin ; calcium ; mineralisation ; tooth germ

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Physik

Notizen: Summary Calmodulin, a calcium binding protein, has been implicated in the regulation of many calcium-dependent biological processes. Since calcium has an important role in hard tissue genesis, both at intra- and extracellular levels, we anticipate that calcium binding proteins may modulate this process. The present study investigated a mineralising tissue, the rat molar tooth germ, to determine the presence of calmodulin-like activity. A heat-treated cell-free extract of tooth germs provided enhancement of Ca2+-dependent Mg2+-ATPase and 3′:5′-nucleotide phosphodiesterase activity. No enhancement occurred in the absence of calcium or in the presence of trifluoperazine. SDS-polyacrylamide gel electrophoresis of this extract revealed a protein band of approximately 18,000 mol. wt. These findings indicate the presence of calmodulin-like activity in rat molar tooth germs and support the proposal that calcium and calcium binding proteins, in particular calmodulin, have a major regulatory role in the biology of mineralising tissues.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02409487

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Digitale Medien

Cloning and partial characterization of the cDNA encoding the fox sperm protein FSA-Acr.1 with similarities to the SP-10 antigen (1995)

Beaton, Sandra ; Have, José ten ; Cleary, Andrew ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Molecular Reproduction and Development 40 (1995), S. 242-252

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ISSN: 1040-452X

Schlagwort(e): Spermatozoa ; Antigens ; Acrosome ; Immunocontraceptive vaccine ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: We have isolated and characterized a cDNA, cFSA-Acr.1, encoding a testis-specific fox sperm antigen. The antigen is located on the inner acrosomal compartment, and is expressed during spermatogenesis on the developing acrosome of round and elongating spermatids. Database searches with the deduced amino acid sequence of cFSA-Acr.1 revealed that the clone has high homology to both human and baboon sperm protein SP-10, and the mouse sperm protein, MSA-63. The region of highest homology is within the carboxyl terminus. In the middle of the open reading frame, the fox sequence shows unique sequences absent from both the human, baboon SP-10, and mouse MSA-63 sequences. In addition to cFSA-Acr.1, two other clones were also isolated from the same fox testis cDNA library, and sequence analysis shows that they may represent alternatively spliced mRNAs coding for other FSA-Acr proteins. © 1995 Wiley-Liss, Inc.

Zusätzliches Material: 11 Ill.