ISSN:
1432-2048
Keywords:
ATP synthase
;
Cytochrome f
;
Immuno-gold labelling
;
Light-harvesting chlorophyll a/b protein
;
Ribulose 1,5-bisphosphate carboxylase/oxygenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The proteins ribulose 1,5-bisphosphate carboxylase/oxygenase, ATP synthase, light-harvesting chlorophyll a/b protein, and cytochrome f, have been localized in mesophyll chloroplasts of barley (Hordeum vulgare L.) by electron microscopy of immunogold-labelled sections. The light-harvesting chlorophyll a/b protein and cytochrome f are shown to be present in the grana, both within the stacks and at the margins, and in the stromal membranes. Although the absolute amount of labelling for these proteins is greater in the grana than in the stromal membranes, when expressed as label/membrane length the partitioning appears approximately equal between appressed and non-appressed membranes for both the light-harvesting chlorophyll a/b protein and cytochrome f. ATP synthase is restricted to the non-appressed thylakoid membranes, and ribulose 1,5-bisphosphate carboxylase/oxygenase is uniformly distributed through the stromal contents.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392229
Permalink
