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  • 1
    ISSN: 1432-2242
    Keywords: Embryo rescue ; Glycine spp. ; Intersubgeneric hybrids ; Soybean ; Wide hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Employing a different culture strategy, we obtained a greatly improved frequency of embryo rescue in intersubgeneric soybean hybrids. Successful crosses were obtained in 31 different genotype combinations between nine Brazilian soybean lines as the female parents and 12 accessions from Glycine canescens, G. microphylla, G. tabacina and G. tomentella. The hybrid pod retention rate dropped to about 10% during the first 8 days after pollination and stayed largely unchanged up to the 20th day. Immature harvested seeds fell into three size groups: Group 1, smaller than 1.3 mm (mostly empty seed coats); Group 2, 1.9–5.0 mm; Group 3, larger than 5 mm (from selfing). A total of 90 putative hybrid embryos were rescued using a highly enriched B5 medium to nourish the newly dissected embryos. The growing embryos were then placed in a high osmotic, modified B5 medium to induce maturation and dormancy. Schenk and Hildebrandt medium was used to germinate the dormant, partially dehydrated, physiologically mature embryos. Approximately 37% of the rescued embryos developed into plantlets in vitro, and approximately 8% grew into mature plants in the greenhouse. Morphological, cytological and isoenzyme patterns confirmed the hybrid status of all seven mature plants, all of which were generated using G. tomentella G 9943 as the paternal parent. It was observed that all soybean lines crossed with G 9943 were capable of producing mature hybrid plants. There was no correlation between the initial size of Group 2 seeds and plant survival rate. The hybrids were cloned by grafting and treated with colchicine. One of the treated plants displayed chromosome doubling.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Natural hazards 22 (2000), S. 269-294 
    ISSN: 1573-0840
    Keywords: magnitude scales ; magnitude-intensity relationships ; uncertainties ; magnitude assignments ; Ibero-Maghrebian region ; historical earthquakes ; isoseismal maps ; earthquake catalogues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract In this paper we present magnitude (Ms) – magnitude(mb) and magnitude-intensity relationships which areconsidered the most adequate in the Ibero-Maghrebianregion. This work is based on selected samples ofrecently revised events with magnitude mb assigned bythe Instituto Geográfico Nacional (I.G.N.) and Msassigned by I.S.C and N.E.I.C., and isoseismal mapsfrom 142 events. Using these data, we have obtainedone magnitude (Ms) – magnitude (mb) relationship, twomagnitude (mb and Ms) assignment relationships viaepicentral intensity (I0), and ten magnitude (mb andMs) assignments relationships via macroseismicinformation: four using Ambraseys' methodology (1985)and six using the isoseismal area of degree III, IV and VI. According to the obtained results it could be concluded that historical magnitude assignment with lesser uncertainties are those obtained via macroseismic information using magnitude-intensity relationships with Ambraseys' methodology (1985). The magnitude-isoseismal area assignment relationships have, in most cases, great differences depending on the degree of the isoseismal area used. Magnitude assignments via epicentral intensity have the highest uncertainties. Geographic regionalization of the relationshipshas been studied but the highest correlations and statistical significance are obtained when we fit all the Ibero-Maghrebian region data. Finally we have used the results obtained in this workto assign magnitude to some important historicalearthquakes in the Ibero-Maghrebian region: the 1755Lisbon earthquake, the 1680 Málaga earthquake, the1829 Torrevieja earthquake and the 1884 Arenas del Reyearthquake. According to our relationships andmethodology we have assigned an Ms value of 9.3 ±0.6 to the 1755 Lisbon earthquake (its mb magnitudecannot be estimated due to the saturation of the mbscale), an mb value of 6.3 ±0.4 and an Ms valueof 6.9 ± 0.6 to the 1829 Torrevieja earthquake, anmb value of 6.2 ± 0.4 and an Ms value of 6.4 ±0.6 to the 1680 Málaga earthquake and an mb valueof 6.1 ± 0.4 and an Ms value of 6.5 ± 0.6 tothe 1884 Arenas del Rey earthquake.
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