ALBERT

Description: The Multispectral Scanner (MSS) was launched on the Earth Resources Technology Satellite (ERTS-1) on July 23, 1972. The MSS has two calibration systems, one internal and one external. Both calibration systems have shown strong, spectrally dependent performance degradation since launch. This paper presents details on the optical system of the MSS and data on the performance degradation as a function of both spectral interval and time in orbit. The history of the MSS during tests is traced, and it is shown that hydrocarbons from an external source may have been deposited on optical surfaces in the instrument. It is postulated that these contaminant coatings may have polymerized as a result of the exposure to UV light from the sun, increasing their blue absorbtion and accounting for the observed performance degradation.

Description: Sun calibration data from Landsat-2 are presented and compared with those from Landsat-1 (ERTS-1). The data support the hypothesis that organic contamination caused the poor performance of the Landsat-1 sun calibration mirror, since extra cleanliness precautions were taken with the Landsat-2 mirror. These precautions are described; of particular importance was the aluminum foil covering kept close to the reflective surfaces of the second mirror during the prelaunch period.

Description: The Multispectral Scanner carried by the ERTS-1 satellite (launched on July 23, 1972) is a very high resolution line scanning imaging radiometer operating in the visible and near infrared spectral regions. The instrument contains optical surfaces that are exposed to both direct and reflected sunlight, and the reflectivity and transmission of some of these surfaces have decreased in orbit. Details of the optical system are explained, and data on the degradation are reviewed as a function of both spectral interval and time in orbit. The history of the scanner during testing (when hydrocarbons from an external source may have been deposited on the optical surfaces) is also examined, and a mechanism is postulated for the observed degradation in orbit.

Description: Dimethyl sulfoxide (DMSO) has been used as a standard cryopreservative agent for mammalian cell culture; however, prolonged exposure of thawed cells to DMSO can alter cell growth. While DMSO is easily eliminated in ground-based experiments, removal of DMSO in flight-based experiments is more difficult due to various on-orbit constraints. Failure of cryopreservation is due to a number of factors, including intracellular ice formation, solute effect, and apoptotic cell death following thawing. One objective of this study is to identify and characterize an alternative cryopreservative that could be used on the International Space Station (ISS). We systematically screened for potential permeating and non-permeating agents using a human colorectal carcinoma cell line, MIP-101. Cells were suspended in cryopreservation solution and frozen either following a two-step procedure involving initial cooling at -1 C/min overnight followed by storage in liquid nitrogen (LN2) vapor, or by freezing cells directly in the LN2 vapor phase at -10 C/min. Ability to preserve cellular function after one cycle of freeze-thawing was assessed by the recovery of viable cells in short and long-term cell culture experiments. Results showed that permeating preservatives glycerol (G) and ethylene glycol (EG) had an efficacy (80-110%) comparable to, if not better than, 7.5% DMSO; but, propylene glycol (PG) had a somewhat lesser efficacy. Among the non-permeating preservatives, trehalose, raffinose, and dextran exhibited significant protective effect (50-80%) relative to that offered by 7.5% DMSO, but at -10 C and not at -1 C/min cooling rate. Preliminary data thus suggest that a combination of permeating and non-permeating agents may have improved efficacy as a cryoprotectant and serve as an alternate to DMSO for experimentation on ISS.