Publication Date:
1998-07-17
Description:
Activation of nonreceptor protein tyrosine kinases (PTKs) is essential for T cell receptor (TCR) responsiveness; however, the function of individual PTK substrates is often uncertain. A mutant T cell line was isolated that lacked expression of SLP-76 (SH2 domain-containing leukocyte protein of 76 kilodaltons), a hematopoietically expressed adaptor protein and PTK substrate. SLP-76 was not required for TCR-induced tyrosine phosphorylation of most proteins, but was required for optimal tyrosine phosphorylation and activation of phospholipase C-gamma1 (PLC-gamma1), as well as Ras pathway activation. TCR-inducible gene expression was dependent on SLP-76. Thus, coupling of TCR-regulated PTKs to downstream signaling pathways requires SLP-76.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yablonski, D -- Kuhne, M R -- Kadlecek, T -- Weiss, A -- CA72531/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1998 Jul 17;281(5375):413-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, Howard Hughes Medical Institute, Box 0795, University of California, San Francisco, San Francisco, CA 94143-0795, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9665884" target="_blank"〉PubMed〈/a〉
Keywords:
*Adaptor Proteins, Signal Transducing
;
Calcium/metabolism
;
Calcium-Calmodulin-Dependent Protein Kinases/metabolism
;
Carrier Proteins/metabolism
;
Cell Line
;
DNA-Binding Proteins/metabolism
;
Enzyme Activation
;
Gene Expression Regulation
;
Humans
;
Inositol Phosphates/metabolism
;
Interleukin-2/genetics
;
Isoenzymes/*metabolism
;
Jurkat Cells
;
*Membrane Proteins
;
Mitogen-Activated Protein Kinase 1
;
NFATC Transcription Factors
;
*Nuclear Proteins
;
Phospholipase C gamma
;
Phosphoproteins/metabolism/*physiology
;
Phosphorylation
;
Phosphotyrosine/metabolism
;
Protein-Tyrosine Kinases/*metabolism
;
Receptors, Antigen, T-Cell/*metabolism
;
Signal Transduction
;
T-Lymphocytes/enzymology/*metabolism
;
Transcription Factors/metabolism
;
Transcriptional Activation
;
Transfection
;
Type C Phospholipases/*metabolism
;
ras Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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