Publication Date:
1991-11-29
Description:
We identified a naturally occurring hepatocyte growth factor (HGF) variant, whose predicted sequence extends only through the second kringle domain of this plasminogen-related molecule. This smaller molecule, derived from an alternative HGF transcript, lacked mitogenic activity but specifically inhibited HGF-induced mitogenesis. Cross-linking studies demonstrated that the truncated molecule competes with HGF for binding to the HGF receptor, which has been identified as the c-met protooncogene product. Thus, the same gene encodes both a growth factor and its direct antagonist.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chan, A M -- Rubin, J S -- Bottaro, D P -- Hirschfield, D W -- Chedid, M -- Aaronson, S A -- New York, N.Y. -- Science. 1991 Nov 29;254(5036):1382-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1720571" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Blotting, Northern
;
Cell Line
;
Culture Media
;
DNA/genetics
;
DNA Replication/drug effects
;
Epidermal Growth Factor/pharmacology
;
Growth Substances/*genetics/isolation & purification/pharmacology
;
Hepatocyte Growth Factor
;
Humans
;
Kinetics
;
Molecular Sequence Data
;
Oligodeoxyribonucleotides
;
Plasmids
;
Poly A/genetics/isolation & purification
;
Polymerase Chain Reaction/methods
;
RNA/genetics/isolation & purification
;
RNA, Messenger
;
Thymidine/metabolism
;
*Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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