Publication Date:
1982-07-30
Description:
Eukaryotic cells contain a set of low molecular weight nuclear RNA's. One of the more abundant of these is termed U2 RNA. The possibility that U2 RNA is hydrogen-bonded to complementary sequences in other nuclear RNA's was investigated. Cultured human (HeLa) cells were treated with a psoralen derivative that cross-links RNA chains that are base-paired with one another. High molecular weight heterogeneous nuclear RNA was isolated under denaturing conditions, and the psoralen cross-links were reversed. Electrophoresis of the released RNA and hybridization with a human cloned U2 DNA probe revealed that U2 is hydrogen-bonded to complementary sequences in heterogeneous nuclear RNA in vivo. In contrast, U2 RNA is not base-paired with nucleolar RNA, which contains the precursors of ribosomal RNA. The results suggest that U2 RNA participates in messenger RNA processing in the nucleus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Calvet, J P -- Meyer, L M -- Pederson, T -- GM 21595/GM/NIGMS NIH HHS/ -- GM 27265/GM/NIGMS NIH HHS/ -- P30 12708/PHS HHS/ -- New York, N.Y. -- Science. 1982 Jul 30;217(4558):456-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6178162" target="_blank"〉PubMed〈/a〉
Keywords:
Base Composition
;
Cell Nucleolus/analysis
;
Cross-Linking Reagents/pharmacology
;
DNA, Recombinant
;
HeLa Cells
;
Humans
;
Nucleic Acid Hybridization
;
RNA/*analysis
;
RNA, Heterogeneous Nuclear/*analysis
;
RNA, Ribosomal/analysis
;
RNA, Small Nuclear
;
Trioxsalen/analogs & derivatives/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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