ISSN:
1617-4623
Keywords:
Recombination
;
Strand exchange
;
Human fibroblasts
;
HeLa cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An activity that can promote homologous pairing and strand transfer between suitable DNA substrates has been partially purified from human skin fibroblasts and from Hela cells. The strand transfer reaction was investigated with DNA substrates consisting of single-stranded circular and duplex linear phage DNA. It requires ATP, and under optimal conditions yields heteroduplex molecules containing one strand from each parental DNA substrate. The reactions appears to be of the same general nature as those mediated by RecA proteins of Escherichia coli and the Rec1 protein of Ustilago maydis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330415
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