ISSN:
1573-5001
Schlagwort(e):
Protein
;
Resonance assignment
;
Automated assignment
;
Heteronuclear assignment
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Summary We present ALPS (Assignment for Labelled Protein Spectra), a flexible computer program for the automatic assignment of backbone NMR resonances of 15N/13C-labelled proteins. The program constructs pseudoresidues from peak-picking lists of a set of two-dimensional triple resonance experiments and uses either a systematic search or a simulated annealing-based optimization to perform the assignment. This method has been successfully tested on two-dimensional triple resonance spectra of Rhodobacter capsulatus ferrocytochrome c 2 (116 amino acids).
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00208806
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