ISSN:
1617-4623
Keywords:
Key words P-element vector
;
Protein tagging
;
Heterochromatin
;
Su(var)3-9
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have developed a new modular vector system that facilitates the combination of various DNA fragments as functional modules for P element-mediated transformation of Drosophila melanogaster. The basic vector pP{GS} contains unique sites for 17 restriction enzymes, including three 8-bp cutters, that allow one to combine various promoter elements, cDNAs and genomic DNA fragments, as well as protein tags and selectable marker genes, for a wide spectrum of transgene analyses. With this new vector system we analysed the chromosomal distribution of the Drosophila SU(VAR)3-9 protein tagged with EGFP, using hsp70-cDNA and genomic Su(var)3-9 constructs. We found preferential association of the tagged SU(VAR)3-9 with centric heterochromatin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008659
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