ISSN:
1432-072X
Keywords:
Key wordsBacillus subtilis
;
sigB
;
Chemostat
;
Glucose limitation
;
Slow growth
;
Survival
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Glucose-limited continuous cultures were used to analyze σB activity at decreasing growth rates. Expression of the σB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h–1, and both genes were induced approximately fivefold at a growth rate of 0.1 h–1 as compared to expression at the maximal growth rate. However, maximal σB activity was only reached when the growth stopped as a result of the exhaustion of the carbon and energy source glucose. During glucose-limited growth, increased expression of the general stress regulon at growth rates below 0.2 h–1 did not provide wild-type cells with a growth advantage over sigB mutants. Instead, expression of the stress regulon seems to constitute a significant burden during glucose-limited growth, resulting in a selective growth advantage of the sigB mutant as compared to the wild-type at a growth rate of 0.08 h–1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050731
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