ISSN:
1432-0983
Keywords:
S. cerevisiae
;
Homothallism/heterothallism
;
HO gene disruption
;
G418 resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho::neo allele yielded G418-resistant yeast transformants in which one of the HO alleles was replaced by the disrupted ho::neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistant transformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho::neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Souther-blot analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00310889
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