Publication Date:
2014-01-18
Description:
The human immunodeficiency virus (HIV) hijacks the endosomal sorting complexes required for transport (ESCRT) to mediate virus release from infected cells. The nanoscale organization of ESCRT machinery necessary for mediating viral abscission is unclear. Here, we applied three-dimensional superresolution microscopy and correlative electron microscopy to delineate the organization of ESCRT components at HIV assembly sites. We observed ESCRT subunits localized within the head of budding virions and released particles, with head-localized levels of CHMP2A decreasing relative to Tsg101 and CHMP4B upon virus abscission. Thus, the driving force for HIV release may derive from initial scaffolding of ESCRT subunits within the viral bud interior followed by plasma membrane association and selective remodeling of ESCRT subunits.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Van Engelenburg, Schuyler B -- Shtengel, Gleb -- Sengupta, Prabuddha -- Waki, Kayoko -- Jarnik, Michal -- Ablan, Sherimay D -- Freed, Eric O -- Hess, Harald F -- Lippincott-Schwartz, Jennifer -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2014 Feb 7;343(6171):653-6. doi: 10.1126/science.1247786. Epub 2014 Jan 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24436186" target="_blank"〉PubMed〈/a〉
Keywords:
Endosomal Sorting Complexes Required for Transport/*metabolism
;
HIV Infections/*virology
;
HIV-1/metabolism/*physiology
;
Humans
;
Imaging, Three-Dimensional/methods
;
Microscopy/methods
;
Protein Subunits/metabolism
;
Virion/metabolism/*physiology
;
*Virus Assembly
;
Virus Release
;
gag Gene Products, Human Immunodeficiency Virus/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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