ISSN:
1573-5001
Keywords:
J coupling constants
;
Isotopic labeling
;
RNA
;
Oligonucleotide structure
;
v0-v4 angle
;
β,ε-angle
;
HCCH-E.COSY
;
P-FIDS-HSQC
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles β and ε. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle χ.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00404274
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