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  • 1
    Electronic Resource
    Electronic Resource
    Electrophoretic and serological comparisons of pathogenesis-related (b) proteins from different plant species (1983)
    Springer
    European journal of plant pathology 89 (1983), S. 293-303 
    Details
    ISSN: 1573-8469
    Keywords: Gynura aurantiaca ; Lycopersicon esculentum ; Malus sylvestris ; amphidiploïdNicotiana glutinosa x Nicotiana debneyi ; Nicotiana sylvestris ; Nicotiana tabacum ; Phaseolus vulgaris ; Vigna sinensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Preparations of pathogenesis-related (b) proteins (PRs) from differentNicotiana species, tomato,Gynura aurantiaca, bean, and cowpea were compared to each other and to bean chitinase and a constitutive apple agglutinin by electrophoresis in polyacrylamide gels both in the absence and in the presence of SDS, and by serological double diffusion analysis using antisera against tobacco PRs and bean chitinase. PRs from different plant genera displayed a similar but not identical range of relative mobilities in both native and SDS gels, whereas bean chitinase and apple agglutinin were clearly different. None of the antisera reacted with any of the PR preparations from plant genera other than the one from which the antigen(s) had been derived. Whilst PRs within the genusNicotiana are serologically related and can be identical, PRs from different plant genera seem to be sufficiently different to be considered as genus-specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01995264
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  • 2
    Electronic Resource
    Electronic Resource
    Somatostatin-containing D cells exhibit immunoreactivity for rat somatostatin cryptic peptide in six mammalian species (1986)
    Springer
    Cell & tissue research 246 (1986), S. 197-204 
    Details
    ISSN: 1432-0878
    Keywords: Electron immunocytochemistry ; Gastro-entero-pancreatic endocrine system ; Gut ; Pancreas ; Prosomatostatin ; Rat somatostatin cryptic peptide (RSCP) ; Rat somatostatin-28
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antisera raised against rat somatostatin cryptic peptide (RSCP; corresponding to amino acids 63–77 of rat pro-somatostatin), somatostatin-28-(1–12) and somatostatin-28-(17–28) were used to compare the morphological distribution of these pro-somatostatin-derived sequences within the gastroenteropancreatic system of six mammalian species, including man. Using the immunogold staining procedure, RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivity was found to be present in all the D cells of the tissues investigated. Extra-islet RSCP and SS28-(1–12) immunoreactive cells were also identified in some species. RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivities were also present in a single case of human duodenal somatostatinoma. Immunostaining of serial ultrathin sections from all specimens in this study revealed that RSCP and both somatostatin immunoreactivities were co-localised in a majority of the reactive cells. Corroborative evidence was obtained by double immunogold staining which further showed that RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivities were co-localised to individual secretory granules in D type cells, both normal and tumour. RSCP and SS28-(17–28) immunoreactivities were invariably co-localised, whereas SS28-(1–12) immunoreactivity was restricted to a sub-population of secretory granules. Our findings suggest that RSCP immunoreactivity is conserved in a number of mammalian species and is stored in each secretory granule type. Consequently, detection of the RSCP sequence may serve as a useful marker for somatostatin-producing systems throughout the diffuse neuroendocrine system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219018
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