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  • 1
    ISSN: 1432-0878
    Keywords: Dictyosome-like structures ; Galactosyltrans-ferase ; Golgi apparatus ; Testis ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal enzymes arylsulfatase and latent acid phosphatase were present in the DLS fractions as were the Golgi apparatus activities thiamine pyrophosphatase and nucleoside diphosphatase. The presence of the latter two enzymes in DLS, plus NADH-ferricyanide reductase, has been verified from cytochemistry. On the other hand, the Golgi apparatus marker, galactosyltransferase, was not enriched in DLS fractions and appeared to be absent. This latter finding, verified from cytochemistry with isolated DLS fractions and, in situ, from [3H]galactose incorporation by testis tubules with analysis by autoradiography, provides the first clear biochemical characteristic that serves unequivocally to distinguish DLS from conventional Golgi apparatus.
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  • 2
    ISSN: 1432-0878
    Keywords: Dictyosome-like structures ; Golgi apparatus ; Guinea pig ; Spermatocytes ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Structures superficially resembling dictyosomes of Golgi apparatus are present in guinea pig spermatocytes and in spermatids in late stages of development. They coexist with Golgi apparatus. In this report, we demonstrate that the dictyosome-like structures (DLS) and Golgi apparatus share cytochemical “markers”, inosine diphosphatase and thiamine pyrophosphatase. Additionally, the cytochemical marker for the mature face of conventional Golgi apparatus as well as most plasma membranes, a glutaraldehyde-resistant NADH-ferricyanide reductase, is present on DLS. The latter reaction is also given by the membranes of the acrosome and that portion of the conventional Golgi apparatus (i.e., the thick cisternae) presumed to be responsible for acrosome formation. A distinguishing feature between DLS and Golgi apparatus is in the distribution of reaction product. Golgi apparatus reaction product is concentrated toward one face of each dictyosome while DLS reaction product is usually randomly distributed across the stacks.
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  • 3
    ISSN: 1432-0878
    Keywords: Dictyosome-like structures ; Golgi apparatus ; Phosphatidylcholine ; Laminated figures ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dictyosome-like structures (DLS) of guinea pig spermatocytes, when prefixed in mixtures of glutaraldehyde and tannic acid, exhibited laminated figures with a repeating periodicity of about 4.5 nm in the spaces between DLS saccules or in association with the surfaces of the DLS saccules. These laminated figures were similar to those figures derived from saturated lipids in other tissues. Alternatively, spaces between saccules were collapsed leaving only thin, electron-dense material separating adjacent saccules. These changes were not observed when the DLS were prefixed in glutaraldehyde before exposure to tannic acid. The presence of laminated figures following fixation with tannic acid and osmium tetroxide suggests that saturated lipids are present in, or associated with, the intersaccular regions of the DLS. The distribution of laminated figures in other membrane structures was not affected by post fixation with tannic acid nor were laminated figures comparable to those of the DLS observed between cisternae of the Golgi apparatus. These results support previous conclusions that DLS are distinct from Golgi apparatus and are a unique component of the germ cell cytoplasm.
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